Cloning of a Heat-stress-associated Protein Hsa32 Gene from Wheat

doi:10.7668/hbnxb.2008.06.001

Abstract

Abstract: Hsa32 (heat-stress-associated 322kDa protein) is mainly found in land plants and takes part in acquired thermotolerance in plants. A novel Hsa32 protein gene was cloned from wheat (Triticum aestivum L.) after heat stress 1 h at 37℃using RT-PCR and RACE(rapid amplification of cDNA ends) technology,and named as TaHsa32. The complete open reading frame (ORF)of the TaHsa32cDNA was 885 bp and encoding a protein of 294 amino acid residues. The comparison of the deduced amino acid sequences of the TaHsa32 with OsHsa32 (from rice),AtHsa32 (from Arabidopsis) and LeHsa32 (from tomato) showed that this was a highly conserved protein and the identity was 83 %,66%,63%,respectively. Using Northern blot analysis it was found that the expression of TaHsa32 gene was a induced by heat stress.

Key words: Wheat, Heat shock protein, Hsa32, Cloning

CLC Number:

Q945

LI Guo-liang, ZHANG Hong-mei, ZHOU Ren-gang. Cloning of a Heat-stress-associated Protein Hsa32 Gene from Wheat[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(6): 1-5. doi: 10.7668/hbnxb.2008.06.001.

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