Abstract: The degenerate primers were designed based on the published information of sequence of Solanaceae plants.The complete long cDNAs encodingCHSwas isolated from wild potatoes(Solanum pinnatisectum)by RT-PCR with the degenerated primers.The sequence analysis indicated thatCHScDNA encoded an polypeptide composed of 389 amino acid residues,the deduce protein showed 89%-93% identity withCHSof Solanaceae plant,and the multiple alignment and phylogenetic analysis demonstrated that it belong to a member of the correspondingCHS-subfamily enzymes;The spatial expression patterns ofCHSgene in wild potato were examined by semi-quantitative RT-PCR with the specific primers.The expression analysis indicated thatCHSgene were preferentially expressed in flowers and stolons,it's tran-scripts could not detected in roots and tubers,and these were involved in accumulation of anthocyanins in slightly purple flowers and stolons,and no accumulation of anthocyanins in white roots and tubers.The response ofCHSgene expression was investigated by RT-PCR analysis in wild potato tubers to white light.The transcripts ofCHSgene were barely present or non-detected in the tuber skins before light induction,but light treatment increased their expression greatly.Expression ofCHSwas highest at 3 days after light exposure,and did not detected at 0.days after light exposure only,while tubers were only white before light treatment,it was indicated that the transcriptional activity ofCHSbasically correlate to antho-cyanin production in tuber skins.

Key words: Chalcone synthase, Anthocyanins, cDNA cloning, Expression pattern, Solanum pinnatisectum

CLC Number: 

• S532.01