Characterization of cry Gene from a Novel Bacillus thuringiensis CY1

doi:10.7668/hbnxb.2007.04.042

Abstract

Abstract: A novel strain CY1 of Bacillus thuringiensis (Bt) was isolated from the soil of Hebei province by Sodium acetate-antibiotic method. Bipyramidal inclusions could be visualized by microscope. SDS-PAGE analysis indicated that the strain producing a 130 kDa protein. The cry gene analysis of the strain was based on multiplex PCR with novel general primers that could detect the cry1, cry11, cry7, cry2, cry3, cry4/cry10, cry5, cry6, cry7, cry8, cry9, cry11, cry13/1, cry16/17, cry18, cry19, cry21, cry22, cry2/25, cry26/28, cry27/29, cry30, cry32, cry3/35, and cry40 genes. Only cry7 gene were found. PCR product of cry7 gene was sequenced. The cry7 gene fragments had 99% homology to cry7Ab1. The strains were further characterized to carry cry7Ab gene by additional PCRs with specific cry7Ab primers.

Key words: Bacillus thuringiensis, CY1 strain, cry7 gene

CLC Number:

S432

WU Hui-xian, SONG Ping, WANG Qin-ying, SU Xu-dong. Characterization of cry Gene from a Novel Bacillus thuringiensis CY1[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2007, 22(4): 180-183. doi: 10.7668/hbnxb.2007.04.042.

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