Isolation of Mitochondrial DNA and RAPD Analysis with Three Cytoplasmic Male Sterilities in Chinese Cabbage

doi:10.7668/hbnxb.2007.04.029

Abstract

Abstract: Isolation mitochondrial DNA (mtDNA) is the first step for studying molecular characteristics in cytoplasmic male sterile Chinese cabbage (Brassica campestris L. ssp. pekinensis). A simple and economical method was introduced in this article. Green leaves were used without adding DNase I enzyme to eliminate nuclear DNA in it. 11 mtDNAs in Chinese cabbage (including maintainers and 3 cytoplasmic male sterilities: Ogu CMS, Pol CMS and CMS96) were isolated by it. Meanwhile, through RAPD technique, 10 special fragments linked to Ogu CMS and CMS96 were found. 3 special fragments linked to Ogu CMS were found. It primarily proved that the male molecular characteristics of Ogu CMS and CMS96 in Chinese cabbage had similarities as well as differences.

Key words: Chinese cabbage, Cytoplasmic male sterility, mtDNA, Isolation method, RAPD

CLC Number:

S634.1

ZHANG De-shuang, WANG Guo-chen, ZHANG Feng-lan, WANG Yong-jian, FANG Zhi-yuan, YU Yang-jun, ZHAO Xiu-yun, XU Jia-bing. Isolation of Mitochondrial DNA and RAPD Analysis with Three Cytoplasmic Male Sterilities in Chinese Cabbage[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2007, 22(4): 124-129. doi: 10.7668/hbnxb.2007.04.029.

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