Detection of a RAPD Marker Linked to rht Gene in Wheat (Triticum aestivum L.)

doi:10.3321/j.issn:1000-7091.2000.01.002

Abstract

Abstract: Four near-isogenic lines which contained rht,Rht1,Rht2,Rht3 respectively were used for polymorphic analysis at molecular level by RAPD technique. Out of 296 10-mer oligonucleotide primers screened, 25 showed polymorphism among near-isogenic lines. During 6 repeat tests of these primers, 18 could not give the repeat results, 6 could repeat 2 to 3 times, only primer OPAM01 showed the stable results in which a 1.86 kb additional band was found in rht line. OPAM01₁₈₆₀ could be identified as a RAPD marker linked to rht gene in wheat.

Key words: Triticum aestivum L, Gene marker, rht gene, RAPD

CLC Number:

S512.103

GUO Beihai, ZHANG Yanmin, LI Hongjie, WANG Zining, SHI Yunsu, ZHANG Zhongting, LI Songtao, WANG Bin, DU Liqun, ZHU Zhiqing. Detection of a RAPD Marker Linked to rht Gene in Wheat (Triticum aestivum L.)[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2000, 15(1): 7-11. doi: 10.3321/j.issn:1000-7091.2000.01.002.

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