Cloning and Sequence Analysis of HKT1 Promoter in Wheat

doi:10.3969/j.issn.1000-7091.2012.z1.001

Abstract

Abstract: Using specific primers based on wheat HKT1 cDNA sequence ( GenBank accession: U16709) ， singleBAC clone containing HKT1 genomic sequence was identified by screening Aibai Chinese Spring BAC library． 4 192bp HKT1 5'-flanking region was isolated by sequencing BAC plasmid． Nine transcription start sites were predictedby Neural Network Promoter Prediction( NNPP) program analysis． The functional elements were analysed by PLACEprogram． HKT1 promoter region contains the basic elements: TATA-box，CAAT-box，and stress-induced elements:salt-response element，cold-，dehydration-，ABA-and frozen-response elements，WUN-response elements and tissuespecificelements． Cloning of HKT1 promoter showed that it is a feasible method to isolate gene promoter by screeningBAC library and sequencing BAC plasmid in wheat．

Key words: Triticum aestivum L．, High-affinity potassium uptake transporter( HKT1), Promoter

CLC Number:

S512.1

LI Meng-jun, GAO Xin-na, FU Xiao-yi, SHI Zhan-liang, GUO Jin-kao. Cloning and Sequence Analysis of HKT1 Promoter in Wheat[J]. ACTA AGRICULTURAE BOREALI-SINICA, doi: 10.3969/j.issn.1000-7091.2012.z1.001.

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