Cloning and Analysis of Osmotic-stress Induced Gene TaMYBSM151 from Common Wheat

doi:10.7668/hbnxb.2014.04.004

Abstract

Abstract: In order to determine the role of transcriptional factors in drought-resistant molecular mechanism of common wheat,Affymetrix GeneChip Wheat Genome Array was used to analyze the expression-profile of common wheat(Shimai 15)root and leaf under osmotic stress.An osmotic-stress induced EST sequence was identified(GenBank accession No.BJ264503)in the expression-profile chip.One BAC clone was isolated from Shimai 15 genome BAC library using primer pairs designed based on EST-BJ264503 sequence.The MYB transcriptional factor gene(TaMYBSM151)and its 5'-flanking sequence were cloned by sequencing walking using BAC clone plasmid as template.The open reading frame of TaMYBSM151 is 936 bp long coding for a putative 311-amino-acid R1-MYB transcriptional factor. TaMYBSM151 contained three exons and two introns.In 5'-flanking sequence of TaMYBSM151,33 dehydration-response cis-elements were predicted.Real-time RT-PCR revealed that the expression of TaMYBSM151 was induced by osmotic stress,but showed different expression patterns.The results above indicated TaMYBSM151 may be a dehydration-response gene.

Key words: Triticum aestivum L., MYB transcriptional factor, Osmotic-stress induced gene

CLC Number:

S512.3
S330

LI Meng-jun, SHI Zhan-liang, GUO Jin-kao. Cloning and Analysis of Osmotic-stress Induced Gene TaMYBSM151 from Common Wheat[J]. journal1, 2014, 29(4): 19-24. doi: 10.7668/hbnxb.2014.04.004.

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