ACTA AGRICULTURAE BOREALI-SINICA ›› 2014, Vol. 29 ›› Issue (1): 98-102. doi: 10.7668/hbnxb.2014.01.019

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QTL Mapping of Root and Shoot Traits at Seedling Stage in Barley

LUAN Hai-ye1,2, Lv Chao1, ZHANG Xin-zhong1, CHEN He2, CHEN Jian2, SHEN Hui-quan2, TAO Hong2, QIAO Hai-long2, ZANG Hui2, XU Ru-gen1   

  1. 1. College of Agriculture, Yangzhou University, Yangzhou 225009,China;
    2. Tnstitute of Agricultural Science in Jiangsu Coastal Areas, Yancheng 224002, China
  • Received:2013-08-11 Published:2014-02-28

Abstract: A population of DH was constructed,which derived from a cross between Taixing 9425 with waterlogging tolerance and Franklin which is susceptible to waterlogging. The five characters associated with waterlogging tolerance including maximum root length,root fresh weight,root dry weight,shoot fresh weight,shoot dry weight were investigated under water stress. Transgressive segregations for all traits were observed in the population,and their frequencies were showed a continuous distribution. The characters were controlled by multi-genes with major effects, and minor modifiers. Based on the linkage map,the composite interval mapping approach of QTL Cartographer 2. 5 was used to detect the QTL for these traits. A total of 13 QTL associated with root and shoot traits were mapped on all chromosomes except 2 and 3. All QTL were responsible for interpreting 10. 99%- 20. 7%,and the range of LOD were 2. 59- 3. 83. Among these QTL,2 QTL controlling maximum root length,2 QTL controlling root fresh weight, 3 QTL controlling root dry weight,3 QTL controlling shoot fresh weight and 3 QTL controlling shoot dry weight were detected. Tightly linked QTL regions on chromosome 6 and 7 were identified,which suggested the existence of pleiotropic or genetic linkage. The above result played a vital role in marker assisted selection and fine mapping of QTL associated with root and waterlogging tolerance.

Key words: Barley, Seedling stake, Double haploid line population, Quantitative trait locus, Root and shoot traits

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Cite this article

LUAN Hai-ye, Lv Chao, ZHANG Xin-zhong, CHEN He, CHEN Jian, SHEN Hui-quan, TAO Hong, QIAO Hai-long, ZANG Hui, XU Ru-gen. QTL Mapping of Root and Shoot Traits at Seedling Stage in Barley[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2014, 29(1): 98-102. doi: 10.7668/hbnxb.2014.01.019.

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