Tissue Culture and Plant Regeneration of Barley Immature Embryos Bombed by Microprojectile

doi:10.3321/j.issn:1000-7091.2003.04.001

Abstract

Abstract: Effects of different ABA,2,4-D concentrations and ZT and IAA ratio on callus induction and plant regeneration of barley immature embryos,bombed by microprojectile,were studied.The results demonstrated that 1.0 mg/L ABA could effectively inhibit coleoptile growth of immature embryo without negative effects.Frequency of bud differentiation was up to 8%～17% from embryonic callus induced by the treatment of 1.0 mg/L 2,4-D,while few buds induced by 4.0 mg/L 2,4-D.Desirable combination of ZT and IAA for bud differentiation was 1.0 mg/L ZT and 0.1 mg/L IAA.According to optimum hormone concentrations,plant regeneration frequency of bombed immature embryos from six barley varieties were at a range of 2%-8%.

Key words: Microprojectile, Barley immature embryos, Hormone, Callus, Plant regeneration

CLC Number:

S512.3

LIU Lei, YIN Jun, REN Jiang-ping, LI Hui-yong, LI Lei. Tissue Culture and Plant Regeneration of Barley Immature Embryos Bombed by Microprojectile[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2003, 18(4): 1-4. doi: 10.3321/j.issn:1000-7091.2003.04.001.

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