rotoplast Culture and Plant Regeneration in Upland Cotton

doi:10.3321/j.issn:1000-7091.1999.01.015

Abstract

Abstract: In callus induction of Gossypium hirsutum L.cv.coker 201, IAA and 2, 4-D showed positive effect and KT showed negative effect, while negative effect was shown with the three hormones in suspensions.The best enzymatic combination in protoplast isolation from embryogenetic suspensions was 3% cellulase(Onozuka R10)+1.5%pectinase, and the best hormone combination in protoplast culture was IAA 0.5mg/L+KT 0.1mg/L. Appropriately increasing protoplast density benefited protoplast culture. Light and temprature affected the protoplast culture.Protoplast-derived calli growed rapidly in suspension culture medium without any hormone and regenerated plants through differentiation culture.

Key words: Gossypium hirsutum L, Protoplast culture, Plant regeneration

LÜ Fubing, Zhang Xianlong, Liu Jinlan. rotoplast Culture and Plant Regeneration in Upland Cotton[J]. ACTA AGRICULTURAE BOREALI-SINICA, 1999, 14(1): 73-78. doi: 10.3321/j.issn:1000-7091.1999.01.015.

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