Improvement of Tissue Culture of Portulaca oleracea L.

doi:10.3321/j.issn:1000-7091.2006.z1.015

Abstract

Abstract: Asepsis plantlets were obtained from seeds of wildness Portulaca oleracea L.. Cotyledon and hypocotyle-donary axis were used as explants to induce calli and differentiate shoots and roots in the MS medium containing 18 different concentrations of 6-BA, 2, 4-D, NAA, and KT. From data obtained the best inducement condition of Portulaca oleracea L. calli tissue: prime medium: MS+3% sucrose+6-BA 1 mg/L+2, 4-D 1 mg/L; shoots proliferation medium: MS+3% sucrose+KT 3 mg/L+NAA 0.5 mg/L; crude root substrate: MS+3% sucrose.

Key words: Portulaca oleracea L., Tissue Culture, Plant Regeneration

CLC Number:

LIU Hong-mei, JIANG Ji-zhi, GONG Wei-bo, LI Li, LIAO Xiang-ru. Improvement of Tissue Culture of Portulaca oleracea L.[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2006, 21(S1): 63-66. doi: 10.3321/j.issn:1000-7091.2006.z1.015.

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http://www.hbnxb.net/EN/Y2006/V21/IS1/63

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