Abstract: Four kinds of explants including seeds, stem segments of seedling, shoot tips with petioles,leaves and axillary bud of eight sweet beets strains were used to study the rapid-multiplication, theregeneration of plantlets, plat transplanting of plantlets in vitro. The results showed: MS+6-BA 0.8-1.Smg/L+NAA 0.2–0.3mg/L is the best medium for regenerated plantlets induction to all kinds of explants, and the plantlet grows robust. Using MS+NAA 1.0–1.5mg/L or MS+NAA 1.0 l.2mg/L+IBA 0.2mg/L as rooting culture media, the rooting rate will be more than 85%, and root is developed, the survival rate of regenerated pla}itlets reaches more than 90%. A series of plantlet regeneration and rapid-multiplication in vitro system of sweet beet has been set up, which can also be used as receptor system for gene transfer.

Key words: subarbeet, Tissue culture, Plantlet regeneration, Rapid-Multiplication

CLC Number: 

• S566.3