Somatic Embryogenesis of Sweet Cherry and Plantlet Regeneration

doi:10.3321/j.issn:1000-7091.2006.z2.031

Abstract

Abstract: Cultivars sweet cherry Hongdeng was used as the materials.An immature pterodia was taken from young fruit disinfected with 0.1% HgCl₂.Shoot multiplication culture and rooting culture were conducted after emergence of seedlings.The result indicates that the embryogenic callus(EC) frequency was 53.1% at 2mg/L of 2,4-D added in MS medium,After subcultured for several times,somatic embryogenesis at high frequency(81.0%) was obtained on MS medium supplemented with 6-BA 1.0mg/L+NAA 0.1mg/L.Somatic embryos can keep on growing and proliferating on appropriate medium.

Key words: Sweet cherry, Callus, Somatic embryogenesis, Plantlet regeneration

CLC Number:

S662.5

WU Ya-qin, ZHAO Yan-hua, LIU Guo-jian, LI Chun-min. Somatic Embryogenesis of Sweet Cherry and Plantlet Regeneration[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2006, 21(S2): 125-128. doi: 10.3321/j.issn:1000-7091.2006.z2.031.

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