Abstract: Using the mature embryos of four winter wheat cultivars to be the explants, the effects of pre-treatments of temperature, 2, 4-D concentrat ions in callus format ion medium, and phytohormone types and their combination in callus differentiation medium on callus formation and differentiation were studied. The results showed that low temperature (4℃) pre-treatment could obviously improve the callus formation rate compared to room temperature pretreatment (25℃). Suitable 2, 4-D concentrat ion for callus formation in the callus induction medium was 4 mg/ L. The combination of phytohormones of 1 mg/L KT + 2 mg/L 6-BA had higher the callus differentiation rate than those in 1 mg/ L KT or in 2 mg/ L 6-BA, the single type of phytohormone treatment. Under the same culture condit ion, there were dramatic differences on callus formation and differentiation among the tested cultivars. Thus, low temperature pretreatment, using suitable 2, 4-D concentration during the callus induction process, combining the suitable phytohormone combination in callus differentiation are important factors, to improve the callus formation and differentiat ion, when using the mature embryo to be the plant regeneration explants.

Key words: Winter wheat, Mature embryo, Callus, Differentiat ion

CLC Number: 

• S512.1