Abstract: Three varieties( C. IL, PURPLE and APOLLO) were applied in somatic embryogenesis of Asparagus off icialis L. Different growth substances such as 2, 4-D, KT, 6-BA and NAA were added in theMS substrate.Results indicated that somatic embryos were induced directly from immature embryo axes cultured on basal MS medium supplemented with 2, 4-D 1. 0mg/ L, KT 015 mg/ L and 6-BA 210 mg/L1When decreasing the level of 2, 4-D, yellow embryogenic ca-l lus were induced from tissue of somatic embryos cultured for 2- 3 times, and somatic embryos at different stages appeared1The embryogenic cell first divided into multicellular proembryos, then globular embryoid, pearshaped embryoid, long embryoid, scutiform embryoid and mature embryoid. Asparagus off icialis L. embryogenesis from epidermical cells or subepidermical cells was similar to that of the zygotic embryo.

Key words: Asparagus off icinalis L., Tissue culture, Somatic embryogenesis, Histocytology

CLC Number: 

• S644.6