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Detection of Glycyrrihizic Acid in Glycyrrihizic Suspension Culture Cell and Herbal Medicine ,Plant

LINan1, GAOGuang-rui2, WEIJing-fang1, LIDong-jie1, LIUYing1, XUEZhi-zhong1   

  1. 1. College of Bioscience and Bioengineering ,Hebei University of Science and Technology , Shijiazhuang 　050018 ,China;
    2. The Science and Technology Department of Hebei Province , Shijiazhuang 　050021 ,China
  • Received:2007-02-20 Published:2007-12-30

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Abstract: By different extractant , glycyrrihizic acid can be extracted from glycyrrihizic roots , stems , leaves , herbalmedicine and Glycyrrihizic suspension culture cells .The crude content of glycyrrihizic acid extracted by 10 %ethanol containing0.5 % ammonia was higher than that extracted by 50 % ethanol .The results showed the content of inammoniumglycyrrhetate optimized glycyrrihizic suspension culture cells were higher than that in other materials .And also by cellsuspension culture , the maximum content of glycyrrihizic acid can reach 160.81 mg/g .

Key words: Glycyrrihizic suspension culture cell, Extractant, Glycyrrihizic acid

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LINan, GAOGuang-rui, WEIJing-fang, LIDong-jie, LIUYing, XUEZhi-zhong. Detection of Glycyrrihizic Acid in Glycyrrihizic Suspension Culture Cell and Herbal Medicine ,Plant[J]. ACTA AGRICULTURAE BOREALI-SINICA, doi: 10.7668/hbnxb.2007.S1.048.

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