Factors Affect Callus Induction from Various Explants of K199

doi:10.7668/hbnxb.2008.S1.026

Abstract

Abstract: The explants used in the study are whole seed, detached mature embryo and leaf segment of wheat cultivar K199. The factors affecting the induction and differentiation of calli were studied. The results showed that mature embry opieces and basal segments of young leaves were the best explant sources for K199. Good callus with high regeneration fre??quency could be obtained from medium containing Kinetin(KT)3 mg/ L and 2, 4 D ichlorophenoxyacetic acid(2, 4 D)0.01 mg/ L.

Key words: Wheat (Triticum aestivum L. ), Tissue culture, Mature embryo, Young leaf

CLC Number:

S512.1

ZHANG Chi, LI Xia, WANG Yu-zhen. Factors Affect Callus Induction from Various Explants of K199[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(S1): 104-108. doi: 10.7668/hbnxb.2008.S1.026.

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