Study on Isolated-microspore Culture and Plant Regeneration of Cauliflower

doi:10.7668/hbnxb.2007.06.013

Abstract

Abstract: The cultivation of isolated microspores with 186 caulfflowers cross cultivate and combinations were studieb. The results showed that genotype was a critical factor affecting embryogenesis in isolated microspore culture. The heat shock pretreatmern at 32 ℃ for 24 h was necessary for embryo formation; the stage from uninuclear to binuclear was the optimum period for embryo inocanent and the highest frequency of anbryogenesis could be attained; it also indicated that the addition of 0.1 mg/ L CUBA and 0.005 mg/L NAA to culture medium could promote embryoid development. The survival rate of 20-day embryo was greatly enhanced when treatebwith dedifferentiation. Consequernly, the ratio of plant re-generation was 53%, the ratio of DHin regenerate plarn was 83.5%.

Key words: Cauliflower, Isolated-microspore culture, Plant regeneration, Double haploid

CLC Number:

S634

ZHAO Qian-cheng, JI Li-zhu, CAI Rong-qi, LIU Li-li, TONG Zhi-qiang, SUN De-ling. Study on Isolated-microspore Culture and Plant Regeneration of Cauliflower[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2007, 22(6): 65-68. doi: 10.7668/hbnxb.2007.06.013.

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http://www.hbnxb.net/EN/Y2007/V22/I6/65

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