Influencing Factors of Shoot Apical Meristem Transformation via Particle Bombardment in Cotton

doi:10.3321/j.issn:1000-7091.2006.z1.008

Abstract

Abstract: The phytase gene (PhyA) was transformed into shoot apical meristems of NDKB18 via microprojectile-bombardment. Some transgenic plants were obtained from these bombarded explant materials. An improved transformation system of meristem via gene-gun bombardment was established based on some influential factors investigated. The results showed that the ratios of regenerated plant were related with the ingredients of regeneration medium, such as hormones, active charcoal and different sugars. The efficiency of transgenic plant and transformation cycle were affected by the bombardment distance, the initiative time of selection, the initiative concentration and concentration gradients of kanamycin in the process of selection.

Key words: Cotton, Shoot apical meristem, Genetic transformation, Gene-gun bombardment, Plant regeneration

CLC Number:

S562

LI Qiu-ling, WANG Xing-fen, MA Zhi-ying, ZHANG Gui-yin. Influencing Factors of Shoot Apical Meristem Transformation via Particle Bombardment in Cotton[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2006, 21(S1): 32-36. doi: 10.3321/j.issn:1000-7091.2006.z1.008.

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