In vitro Culture of Immature Embryos or Ovules and Plantlet Formation in Cotton

doi:10.3321/j.issn:1000-7091.2006.z1.009

Abstract

Abstract: The in vitro culture of immature embryos or ovules was employed in embryo rescue to overcome imcompatible interspecific hybridization in Gossypium L. It was also an important means in studying embryo and fiber development mechanism. The methods of immature embryo (ovule) culture have been improved since 1935, but they were still relatively complicated. Different mediums should be exploited at the stage of embryo development to achieve the embryo maturation and plantlet format ion. BT or MS were usually used as basic mediums. Cotton genotypes markedly influenced the effect of the culture in vitro. Various combinations and concentrations of IAA, KT or GA were usually supplemented in the stages from immature embryo development to embryo maturation. The concentrations of carbon sources, nitrogen sources and combinations of NO₃^-/NH₄⁺ played an important role in the culture process.

Key words: Cotton, Culture of immature embryo or ovule, Plantlet formation, Influencing factor

CLC Number:

S562.01

WANG Yan-xia, WANG Xing-fen, MA Zhi-ying. In vitro Culture of Immature Embryos or Ovules and Plantlet Formation in Cotton[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2006, 21(S1): 37-40. doi: 10.3321/j.issn:1000-7091.2006.z1.009.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.3321/j.issn:1000-7091.2006.z1.009

http://www.hbnxb.net/EN/Y2006/V21/IS1/37

References

[1] Beasley J O. Hybridization of American 26-chromosome and Asiatic 13-chromosome species of Gossypium[J]. J Agri Res, 1940, 60, 175-182.
[2] Mauney J R. The culture in vitro of immature cotton embryos[J]. Bot Gazette, 1961, 3: 205-209.
[3] Mauney J R, Chappell J, Ward B J. Effects of malic acid salts on growth of young cotton embryos in vitro[J]. Bot Gazette, 1967, 128: 198-200.
[4] Joshi P C, Johri B M. in vitro growth of ovules of Gossypium hirsutum[J]. Phytomorphology, 1972, 22: 195-209.
[5] Pundir N S. Experimental embryology of Gossypium arboreum L. and G. hirsutum L. and their reciprocal crosses[J]. Bot Gazette, 1972, 133, 7-26.
[6] Eid A A H, Delange L, Waterkeyn L. in vitro culture of fertilized cotton ovules. 1. The growth of cotton embryos[J]. Cellue, 1973, 69: 361-371.
[7] Beasley C A, Ting I P. The effects of plant growth substances on in vitro fiber development from fertilized cotton ovules[J]. Amer J Bot, 1973, 60 (2): 130-139.
[8] Beasley C A, Ting I P. Effects of plant growth substances on in vitro fiber development from unfertilized cotton ovules[J]. Amer J Bot, 1974, 61 (2): 188-194.
[9] Thengane S, Paranjpe S V, Khuspe S S, et al. Hybridization of Gossypium species through in ovulo embryo culture[J]. Plant Cell Tissue Org Cult, 1986, 6: 209-219.
[10] Borole V K, Dhumale D B, Rajput J P. Embryo culture study in interspecific crosses between G. arboreum and G. hirsuum cotton[J]. Indian J Genet, 2000, 60 (1): 105-110.
[11] Liu C Z, Sun J Z, Liu J L. in vitro interspecific fertilization, embryo development and formation of hybrid seedlings between Gossypium hirsutum and G. arboretum[J]. Euphytica, 1992 60: 79-88.
[12] Mehetre S S, Patil S C, Pawar S V, et al. Ovule embryo cultured hybrid between amphidiploid (Gossypium arboreum Gossypium anomalum) and Gossypium hirsutum[J]. Current Science, 2004, 87 (3): 286-289.
[13] Mehetre S S, Patil S C, Pawar S V, et al. Immature ovule embryo culture-a tool to multiply wild cotton (Gossypium L.) germplasm[J]. Indian J Genet, 2003, 63 (4): 353-354.
[14] Gill M S, Bajaj Y P S. in vitro production of interspecific hybrids in cotton[J]. Current Science, 1984, 53 (2), 102-104.
[15] Gill M S, Bajaj Y P S. Interspecific hybridization in the genus Gossypium through embryo culture[J]. Euphytica, 1984, 33: 305-311.
[16] Gill M S, Bajaj Y P S. Hybridization between diolold (Gossypium arboreum) and tetraploid (Gossypium hirsutum) cotton through ovule culture[J]. Euphytica, 1987, 36: 625-630.
[17] Mirza M A, Shaikh A L. In-ovulo embryo culture of some incompatible species hybrids of Gossypium L[J]. The Pakistan Cottons, 1984, 28 (2), 117-125.
[18] Osama A M. Effect of plant growth regulators on in vitro fiber development from unfertilized and fertilized Egyptian cotton ovules[J]. Plant Growth Regulation, 1998, 25(3): 159-164.
[19] Robert W S, Giavalis S. Pre-and post-anthesis application of exogenous hormones alters fiber production in Gossypium hirsutum L. Cultivar Maxxa GTO[J]. J. Cotton Science, 2004, 8: 105-111.
[20] Beasley C A. Temperature-dependent response to indoleacetic acid is altered by NH₄+ in cultured cotton ovules[J]. Plant Physiol, 1977, 59: 203-206.
[21] 胡绍安, 李秀兰. 棉属二倍体野生棉与四倍体栽培棉种间杂交的胚珠培养[J]. 遗传学报, 1982, 9 (1): 57-62.
[22] Stewart J M, Hsu C L. Hybridization of diploid and tetraploid cottons through in-ovulo embryo culture[J]. J. Heredity, 1978, 69: 404-408.
[23] 张献龙, 孙济中, 刘金兰. 棉属远缘杂种胚珠离体培养研究[J]. 中国农业科学, 1988, 21 (6): 53-58.
[24] 陈志贤, 张淑娥, 山静宜. 棉属种间杂种胚珠培养及成苗技术改进研究[J]. 棉花学报, 1985, 1: 27-33.
[25] Stewart J M, Hsu C L. In ovulo embryo culture and seedling development of cotton (Gossypium hirsutum L.)[J]. Planta, 1977, 137: 113-117.
[26] Bajsj Y P S, Gill M S. in vitro induction of genetic variability in cotton (Gossypium spp.)[J]. Theor Appl Genet, 1985, 70: 363-368.
[27] Mehetre S S, Aher A R. Embryo rescue: a tool to overcome incompatible interspecific hybridization in Gossypium Linn[J]. Indina Bio Tech, 2004, 3 (1): 29-36.

Please choose a citation manager

Content to export