Abstract: :The granular calli of konjac was employed for study of liquid culture and plant regeneration.The results showed that MS +BA 1.0 mg/L +2, 4-D 0.2 mg/L + sugar 30g/L + agar 6.0 g/L couldefficiently induce granularcalli from the bud.Obtained calli were cut about 0.3 cm and then subsequently cultured in MS +BA 0.05mg/L+NAA 0.1 mg/L+sugar 30 g/L +PVP(PolyVinyl-Pyrrolidone)1.0 g/L for establishing liquid culture system.The growth of liquid culture material showed a parabola curve, the weight gain for more than 80% of entire culture period from the sixth day to the twelfth day.In addition, pH value of medium increased during the culture process.The material that was cultured twelve days turned to 1/2MS +BA 0.6 mg/L +NAA 0.1 mg/L +glucose 30 g/L +vitamin C100 mg/L +agar 6.0 g/L for differentiation culture.Big buds that the leaf expects were cut and put on 1/2MS + +NAA 0.1 mg/L + sugar 30 g/L+agar 6.0 g/L for inducing root, eventually the regenerative plantlets were formed.

Key words: Konjac, Liquid culture, Plant regeneration

CLC Number: 

• S632.9