Embryogenesis and Plant Regeneration of Broccoli via Isolated Microspore Culture

doi:10.3321/j.issn:1000-7091.1998.03.020

Abstract

Abstract: Buds of 3 0～5.5mm in length of broccoli (Brassica oleracea var.italica) were removed from the raceme,surface sterilized with full strength commercial bleach (7% sodium hypochlorite)for 15 min and rinsed 3 times with sterile deionized water.Then they were squeezed,filtered,prepared and centrifuged.The density of the suspension was cultured in modified liquid medium 1/2 NLN lacking growth substances.They were sealed with parafilm and incubated in dardness at 32.5℃ for one day and then incubated at 25℃ continually.13 genotypes of broccoli were used in the isolated microspore culture study.Microspore derived embryos were produced in 8 genotypes and 207 microspore derived plants were produced in 5 genotypes. Of the eight genotypes in which microspore derived embryos were gained,the best one was"Balu broccoli"which produced 37.08 embryos per bud.Genotypes influenced isolated microspore culture of broccoli significantly.The addition of active carbon suspension to culture media and the alternation of culture media were also studied.

Key words: Broccoli, Isolated microspore culture, Embryogenesis, Plant regeneration

CLC Number:

S635.303

Zhang Deshuang， Cao Mingqing. Embryogenesis and Plant Regeneration of Broccoli via Isolated Microspore Culture[J]. ACTA AGRICULTURAE BOREALI-SINICA, 1998, 13(3): 102-106. doi: 10.3321/j.issn:1000-7091.1998.03.020.

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