Embryogenesis from IsolatedMicrospore Culture of Chinese Cabbage ( Brassicacampestris L. ssp. chinensis L. )

doi:10.7668/hbnxb.2007.S1.050

Abstract

Abstract: Isolated microspore culture was carried out by ten F1 hybrids of Chinese cabbage ( Brassica campestris L.ssp. chinensis L. ) . It was found that the effect of genotypes on embryoid induction was remarkable. Reducing the contentof major salts of NLN medium by half can raise the embryoid induction rate up to 100% ; the suitable concentrat ion of 6BA and NAA can remarkably enhance microspore embryogenesis.Agarose and active carbon could not only promote em-bryogenesis, but also increase the proport ion of cotyledon-shaped embryoids, the cotyledon-shaped embryoid rate was over90% . 4℃pretreatment of bud for 24 h significantly enhanced frequency of embryoids.

Key words: Chinese cabbage, Isolated microspore culture, Embryoid

CLC Number:

S634.3

WANGChao-nan, FENGHui, JIANGFeng-ying, LIURu-e, ZHOUYing. Embryogenesis from IsolatedMicrospore Culture of Chinese Cabbage ( Brassicacampestris L. ssp. chinensis L. )[J]. ACTA AGRICULTURAE BOREALI-SINICA, doi: 10.7668/hbnxb.2007.S1.050.

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