ACTA AGRICULTURAE BOREALI-SINICA ›› 1994, Vol. 9 ›› Issue (3): 34-38. doi: 10.3321/j.issn:1000-7091.1994.03.008

Special Issue: Wheat Biotechnology

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Callus Inducing Frequency from the Anther of Different Genotypes Winter Wheat and the Electrophoretic Analysis of Proteins in Calli

Yun Yue1, Hu Daofen1, Liu Ming2, Liu Lijun3   

  1. 1. Beijing Plant Cell Bioengineering Laboratory, Beijing 100081;
    2. Institute of Genetics, Academia Sinica, Beijing 100012;
    3. Institute of Crop Breeding and Cultivation, Chinese Academy of Agricultural Sciences, Beijing 100081
  • Received:1993-03-27 Published:1994-09-28

Abstract: The high frequencies of callus induction were obtained from anthers of different geontypes winter wheat on the W142 mediun(a modified W 14 medium).Different genotypes showed significant differences in the frequency of callus induction,IEF and SDS-PAGE analyses on the proteins of wheat anthers and calli thereof were performed with the Phast System Electrophoresis apparatus made by the Phamacia Company.Differences were observed in proteins from the anthers of different genotypes and relationship was considered to exist between the difference and the frequencies of callus induction.No obvious difference was observed in band patterns of anther calli among different genotypes.It was found that there some new protein bands in calli when compared with those of the anthers.This phenomenon probably resulted from the biochemical changes in the process of callus induction.

Key words: Wheat, Genotype, Frequency of callus induction, Electrophoresis

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Cite this article

Yun Yue, Hu Daofen, Liu Ming, Liu Lijun. Callus Inducing Frequency from the Anther of Different Genotypes Winter Wheat and the Electrophoretic Analysis of Proteins in Calli[J]. ACTA AGRICULTURAE BOREALI-SINICA, 1994, 9(3): 34-38. doi: 10.3321/j.issn:1000-7091.1994.03.008.

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