Supercryopreservation of Fruit Tree Pollens

doi:10.3321/j.issn:1000-7091.1993.z1.013

Abstract

Abstract: The pollens from 39 varieties of fruit trees-apple (Mains puinila Mill), pear (Pyrus bretschneideri Rehd) , peach (Primus perisica L. ), plum (P. salicina Lindl) , and cherry (P. avium L. ) were dried for water content to decrease to 10%-20% before being put into liquid nitrogen for a period of time, and then thawed in 40℃ water bath for 1 min. The result showed that the viability and the germination capacity of pollens in vitro treated in this way approached those of fresh pollens. The fresh pollen with 60% of water can not be stored in liquid nitrogen. The water content in pollens is one of the factors influencing the result of fruit storability in liquid nitrogen. If the water content in p Mens is suitable for su-percryopreservation, the process of freezing-thawing of stored polle is can be repeated some times, and the pollen viability can not be influenced. This technology can be used for long-term conservation of fruit pollens.

Key words: Germplasm supercryopreservation, Pollen, Fruit trees

Chen Shuangying, Chang Yongjian, Zhao Yanhua, Zhang Bo, Liu Yu, Zhang Deming. Supercryopreservation of Fruit Tree Pollens[J]. ACTA AGRICULTURAE BOREALI-SINICA, 1993, 8(S1): 60-64. doi: 10.3321/j.issn:1000-7091.1993.z1.013.

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