Electron Microscopic Study on Male Sterile CMS96 and Maintainer Line in Chinese Cabbage

doi:10.3969/j.issn.1000-7091.2012.02.026

Abstract

Abstract: Using transmission and scanning electron microscopy techniques，anther and pollen development, surface of anther and pollen grains were studied in order to obtain differences between CMS96 and maintainer line?in Chinese cabbage(Brassica campestris L． ssp． pekinensis)． Results of transmission electron microscopy showed middle layer and tapetum of CMS96 were separated during meiosis． And early pollen mother cells (PMC) of CMS96?were with irregular shape and many larger gaps． Thus meiosis of PMC was influenced seriously． During mononuclear?stage，microspore began to be vacuolated and mature pollens were empty to be seriously in cell wall and piled together leading to pollen grain abortion． Results of scanning electron microscopy showed pollen grains of CMS96 in?the anther were abnormal and empty with hollow and flat shape． Pollen veins were irregular． No grains were released?because pollen sac closed． Preliminary results showed pollen grains abortion of Chinese cabbage CMS96 began from?meiosis to mature pollen along with the whole pollen development． CMS96 could form pollen grains with cell wall, but the compositions inner the pollen grains were empty． The abnormal pollen grains adhered to the inner anther?without dispersing．

Key words: Chinese cabbage, Male sterile CMS96, Electron microscopy, Pollen grain abortion

CLC Number:

S634.03

ZHANG De-shuang, ZHANG Feng-lan, XU Jia-bing, YU Yang-jun, ZHAO Xiu-yun, WANG Yong-jian, FANG Zhi-yuan. Electron Microscopic Study on Male Sterile CMS96 and Maintainer Line in Chinese Cabbage[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(2): 133-139. doi: 10.3969/j.issn.1000-7091.2012.02.026.

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