Abstract: The seeds from 110 cultivars of wheat were used to examine the factors affecting the efficiency of mature emoryo culture,The experimental results demonstrated:(1)Genotype was a critical factor，which could cause remarkable dif-ferences in callus growth rate and frequency of one一step regeneration from the cultured embryos,(2)Composition of the culture medium also played an important role in the culture, 2，4-D was an essential hormone and had a wide range of usable concentrations, But at excessive levels, it could slow down the growth of callus and seriously inhibit differentiation of organs, 0.5^1 mg/1 NAA or GA in cooperation with 0.5^-1 mg/1 2,4-D was proven beneficial to the culture and resulted in a considerable higher frequency of one-step regeneration, ABA at the same levels promoted only root differentiation,Concentration of sucrose of 3-V7%in the medium was found to be helpful to callus growth.(3)Another factor was the period of callus subculture time, Periods,shorter than one year, could improve callus quality and speed up its proli-feration, On the contrary, longer than one year, resulted in a drastic redu-tion of the regeneration proportion of green plantlets, and an increase in albino plants, However, a high frequency of grccn plant regeneration was obtained by selecting suitable genotypes and ad justing plant hormones in culture medium。

Key words: Wheat, Mature embryo, Callus, Tissue culture