Gene for Tiller Height Regulation and Its Expression Analysis in Sorghum

doi:10.7668/hbnxb.20193558

Abstract

Abstract:

Tiller higher than main stem is one of the important reasons that make the uniformity of sorghum varieties and mechanized production of sorghum complicated.In order to clarify the mechanism of the gene that regulates the tiller height in sorghum,improve the uniformity of sorghum varieties and breed sorghum varieties suitable for mechanized production,based on the mapping results of our previous studies,15 of the sorghum variety whose tiller height was consistent with main stem height as well as 17 of the sorghum variety whose tiller height was higher than main stem height,were selected to form the natural population to have the candidate genes tested.It was found that it was the SNP3 locus belonging to the gene Sobic.009G2133001.v3.2 located in the conserved domains of Hydrolase_4 affects the tiller height,it was named SbTH.With two sorghum varieties K35-Y5 and 1383 taken as material,the expression patterns of gene SbTH in different sorghum tissues were analyzed by Real-time fluorescence quantitative PCR.The results showed that the SbTH gene got expressed in both the roots and the leaves.Though the expression levels were different,the trends were basically the same.In the stem of variety 1383 whose tiller was higher than main stem,the expression level and trend were almost the same.Only in variety K35-Y5 whose tiller height was consistent with main stem height,at anthesis of main stem,reverse expression pattern was observed.While the expression level of main stem reached the maximum,the expression level of tiller was lowered to the minimum.From above results,we concluded that the expression level of SbTH was low in the tiller of K35-Y5,thus the overgrowth of the tiller internodes got controlled,forming the plant phenotype whose stem and tiller had the same height.The expression level of SbTH was high in the tiller of variety 1383,thus the growth of tiller internodes was promoted,which made the tiller be higher than the stem.Therefore,it is believed that the differential expression of gene SbTH at anthesis of main stem is the key to tiller height regulation.

Key words: Sorghum, Tiller height, Quantitative expression

WANG Rui, CHENG Qingjun, WANG Huiyan, JU Lan, PING Jun'ai, ZHANG Fuyao. Gene for Tiller Height Regulation and Its Expression Analysis in Sorghum[J]. Acta Agriculturae Boreali-Sinica, 2023, 38(2): 43-50. doi: 10.7668/hbnxb.20193558.

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References 34

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编号 No.	试验材料 Germplasm tested	分蘖与主茎株高的一致性 Consistency in height of tiller and main stem
1	71077(红)×BCA₂TX623B-2-1	一致
2	泸45 B×3128 B-3-1-3-2	一致
3	CS3541	一致
4	吉16VII27B-1	一致
5	71224红×A2TX623B	一致
6	3401A-3天杂F2-2	一致
7	L黑/吉7031×1383-2-1-1	一致
8	91650×052R-3	一致
9	L07-08 052R×高糯2-1-2C-5	一致
10	91624(红)×周02142-07-1-7-5-3	一致
11	Tx2737×45A-2-1-5R-1	一致
12	湘粱恢×91650-7-2	一致
13	湘粱恢×91650-3-3	一致
14	L黑-4/吉7031	一致
15	741324	不一致
16	L07-08 052R×LR287-31-2-1-2-2	不一致
17	L07-08 052R×南133-1-6	不一致
18	L407B×961547-4-2	不一致
19	TX2737×5-26-3	不一致
20	(961541×0-30红粒变×34-33)-1-13-3-2	不一致
21	吉林糯高粱	不一致
22	红茅糯6号	不一致
23	程NK4420×湘粱恢-3-1	不一致
24	湘粱恢×103R×LR233-3-1	不一致
25	KHCK18 2016	不一致
26	L2381	不一致
27	5-27	不一致
28	忻糯R	不一致
29	天津早糯	不一致
30	朔55-1(N)	不一致
1383	1383	不一致
K35-Y5	K35-Y5	一致

编号 No.	试验材料 Germplasm tested	分蘖与主茎株高的一致性 Consistency in height of tiller and main stem
1	71077(红)×BCA₂TX623B-2-1	一致
2	泸45 B×3128 B-3-1-3-2	一致
3	CS3541	一致
4	吉16VII27B-1	一致
5	71224红×A2TX623B	一致
6	3401A-3天杂F2-2	一致
7	L黑/吉7031×1383-2-1-1	一致
8	91650×052R-3	一致
9	L07-08 052R×高糯2-1-2C-5	一致
10	91624(红)×周02142-07-1-7-5-3	一致
11	Tx2737×45A-2-1-5R-1	一致
12	湘粱恢×91650-7-2	一致
13	湘粱恢×91650-3-3	一致
14	L黑-4/吉7031	一致
15	741324	不一致
16	L07-08 052R×LR287-31-2-1-2-2	不一致
17	L07-08 052R×南133-1-6	不一致
18	L407B×961547-4-2	不一致
19	TX2737×5-26-3	不一致
20	(961541×0-30红粒变×34-33)-1-13-3-2	不一致
21	吉林糯高粱	不一致
22	红茅糯6号	不一致
23	程NK4420×湘粱恢-3-1	不一致
24	湘粱恢×103R×LR233-3-1	不一致
25	KHCK18 2016	不一致
26	L2381	不一致
27	5-27	不一致
28	忻糯R	不一致
29	天津早糯	不一致
30	朔55-1(N)	不一致
1383	1383	不一致
K35-Y5	K35-Y5	一致

引物名称 Primer ID	正向引物(5'-3') Forward primer (5'-3')	反向引物(5'-3') Reverse primer (5'-3')	产物大小/bp Size
SNP1	AAAATCACGTGGATAGGCCA	AGATCCGGCAATGATAGTGG	269
SNP2	CCACTATCATTGCCGGATCT	ATCTGAGGAAGCAAGCGAGA	233
SNP3	GGAGGAAATGCTGTGCTTCT	AAGCGATGGTCCATTGAATC	245
SNP4	TGATTCCTTCGTTGGAGGAG	TCACTAACATTGGGCAACCA	199

引物名称 Primer ID	正向引物(5'-3') Forward primer (5'-3')	反向引物(5'-3') Reverse primer (5'-3')	产物大小/bp Size
SNP1	AAAATCACGTGGATAGGCCA	AGATCCGGCAATGATAGTGG	269
SNP2	CCACTATCATTGCCGGATCT	ATCTGAGGAAGCAAGCGAGA	233
SNP3	GGAGGAAATGCTGTGCTTCT	AAGCGATGGTCCATTGAATC	245
SNP4	TGATTCCTTCGTTGGAGGAG	TCACTAACATTGGGCAACCA	199

引物名称 Primer ID	序列(5'-3') Sequence(5'-3')	退火温度/℃ Annealing temperature
Actin-F	ATTCACGAGACTACCTACAAC	60
Actin-R	ACCAGAGAGGACGATGTT
SNP3-F	GGAGGAAATGCTGTGCTTCT	65
SNP3-R	AAGCGATGGTCCATTGAATC

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