Identification and Function Study in Flowering Time Regulation of PlSEP1 Gene in Tree Peony

doi:10.7668/hbnxb.20193099

Abstract

Abstract:

SEPALLATA1(SEP1) gene is a member of MADS-box gene family which plays an important role in flowering time regulation.In order to clarify the biological function and expression characteristics of PlSEP1 gene in tree peony during flowering,and further understand the mechanism of PlSEP1 gene involved in the regulation of flowering time.A MADS-box family gene,PlSEP1,was screened from the transcriptome data of floral bud development in the reblooming cultivar of tree peony,High noon,the relative expression of PlSEP1 gene in the floral bud development of tree peony was detected by quantitative Real-time PCR,and the function of PlSEP1 gene was explored through over expression in Arabidopsis thaliana.The results showed that,PlSEP1 encoded 244 amino acid with a ORF of 735 bp.The bioinformative analysis showed that PlSEP1 encoded a stable hydrophilic protein,which contained none signal peptide.Phylogenetic tree showed that the PlSEP1 was close with its homologs in Arabidopsis thaliana,Glycine max.Subcellular location assay showed that PlSEP1 protein was located in the nucleus.PlSEP1 expressed highest in flower buds,and reached the highest in the differentiation stage.The phenotype of early flowering in over-expression transgene plant suggested that PlSEP1 regulate flowering time.

Key words: Tree peony, PlSEP1, Floral bud development, Flowering time, Subcellular location

CHANG Yanting, ZHANG Wenbo, XIA Mengsi, FAN Keke, HU Xiaomeng, WANG Shuhua, ZHANG Xue, BAI Yiwei, ZHANG Na, HU Tao, JIANG Zehui. Identification and Function Study in Flowering Time Regulation of PlSEP1 Gene in Tree Peony[J]. Acta Agriculturae Boreali-Sinica, 2023, 38(2): 51-56. doi: 10.7668/hbnxb.20193099.

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Figures/Tables 7

Tab.1 Primer information list

引物Primer	引物序列Primer sequence
PlSEP1-F	GCTCTAGAATGGGAAGAAAAAAAGTGGAGATCAAGCGG
PlSEP1-R	TCCCCCCGGGGAGATCTACACTTACACCATCGACATTCGC
PlSEP1-qpcr-F	CTCTGTGATGCTGATGTGGCTCTCA
PlSEP1-qpcr-R	GCTCTAGCTCCTTCGAATCTAAAGGCC
TUBULIN-qpcr-F	GATGTGTGCTGCTGATCCTCGACAC
TUBULIN-qpcr-R	CTCCTGGATCGAAGTCGAGTTGCC

Fig.1 Multiple sequence alignment of PlSEP1 with other species NaSEP1.Nymphaea odorata;NnSEP1.Nelumbo nucifera;TgSEP1.Tulipa gesneriana;AtSEP1.Arabidopsis thaliana;AcSEP1.Actinidia chinensis; GMSEP1.Glycine max;PcSEP1.Paphiopedilum concolor;DcSEP1.Dendrobium crumenatum.

Fig.2 Phylogenetic tree of PlSEP1 and SEP1 proteins from other species

Fig.3 Subcellular localization of PlSEP1

Fig.4 Relative expression level of PlSEP1 in different tissue Different lowercase letters above bars means significant difference(P<0.05).The same as Fig.5-6.

Fig.5 Relative expression level of PlSEP1 in floral bud development

Fig.6 Early flowering phynotype of OE-PlSEP1 in Arabidopsis

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