Screening and Analysis of BAC Clones Containing Flowering Time Gene COin Brassica campestris L． ssp． pekinensis Olsson

doi:10.3969/j.issn.1000-7091.2012.06.005

Abstract

Abstract: The specific primers designed according to the published Chinese cabbage COgene( Bra008669) were used to screen the Chinese cabbage BAC library through three steps of PCR，and 3 clones containing COgene were obtained． The 3 obtained clones were amplificated and the sequence compared with Chinese cabbage COgene ( Bra008669), the result showed that the amplificated fragments had 99． 73% of homology with the COgene ( Bra008669) and included N-terminal and C-terminal conservated domains．

Key words: Chinese cabbage, Flowering time gene, COgene, BAC clone

CLC Number:

MA Ai-ru, FENG Da-ling, LIU Li-ping, XUAN Shu-xin, WANG Yan-hua. Screening and Analysis of BAC Clones Containing Flowering Time Gene COin Brassica campestris L． ssp． pekinensis Olsson[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(6): 20-23. doi: 10.3969/j.issn.1000-7091.2012.06.005.

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