Expressing and Functional Analysis of GmMYBJ7from Soybean

doi:10.3969/j.issn.1000-7091.2012.06.006

Abstract

Abstract: Plant MYB transcription factors are characterized by the presence of a structurally conserved MYB domain，which involved in the regulation of many aspects of plant growth，development，metabolism and stress responses． A new MYB gene，designated GmMYBJ7( GenBank accession No． DQ902864),was isolated from soybean ( Glycine max). The result of subcellular localization indicated that GmMYBJ7proteins were mostly distributed in the nucleus． Semi-quantitative RT-PCR analysis indicated that the expression of GmMYBJ7would be decreased under the abiotic stresse treatment such as UV-B radiation，drought，and high-salt． GmMYBJ7were transformed into tobacco NC89 with Agrobacterium LBA4404 containing the plant expression vectors pCAMBIA2301-GmMYBJ7． The results showed that the average total flavonoids content decreased significantly in the positive tobacco transformants， which suggested that GmMYBJ7was probably invoved in the basic physiological metabolism by regulating the biosynthesis of flavonoid in plants．

Key words: Soybean, MYB transcription factors, Gene expression and regulation

CLC Number:

Q786

YANG Wen-jie, WU Yan-min, TANG Yi-xiong. Expressing and Functional Analysis of GmMYBJ7from Soybean[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(6): 24-29. doi: 10.3969/j.issn.1000-7091.2012.06.006.

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