MoleculaR Cloning and ExpRession PatteRn of SVP Gene Influencing FloweRing Time in BRassica napus and BRassica juncea

doi:10.3969/j.issn.1000-7091.2013.01.018

Abstract

Abstract: Mutiple homologs of shoRt vegetative phase ( SVP) weRe isolated fRom infloRescences of allotetRaploid Rapeseed cultivaRs Xiangyou No． 15 ( BRassica napus L． ) and Sichuan Yellow-seeded ( BRassica juncea L． ) by using homologous cloning and ReveRse tRanscRiption PCR technology，which was designated BnSVP-1，BnSVP-2，BnSVP-3， BnSVP-4，BnSVP-5 and BjSVP-1，BjSVP-2，BjSVP-3，Respectively． All these sequences have been deposited in Gen- Bank with accession numbeR JQ906717，JQ906718，JQ906719，JQ906720，JQ316471 and JQ906715，JQ906716， JQ316472 Respectively． Results of sequence analysis showed that each homolog was 726 bp and encoded 241 amino acids with typical M，I ，K and C domain of MADS-box gene． The SVP expRession patteRn of Xiangyou No． 15 afteR been tReated with photopeRiod，GA3 spRaying and veRnalization was studied， the Results demonstRated that diffeRent photopeRiods and GA3 spRaying could change its expRession in the stem oR Root，but veRnalization did not． The expRession change would Result in eaRly floweRing． In Rapeseed，SVP expRessed in Root， stem and leaf tissues in vegetable phase， but afteR tRansition to RepRoductive phase its expRession limited only in floRal oRgan such as stamen， pistil and sepal， no expRession was detected in petal． SVP also expRessed in young siliques，while moRe in silique hull than in seed．

Key words: Rapeseed, FloweRing time, SUP gene, Cloning, ExpRession patteRn

CLC Number:

PAN Dan-dan, MAO Qun-fei, ZHANG Jin-shun, YANG Dan, ZHAO Fu-yong,. MoleculaR Cloning and ExpRession PatteRn of SVP Gene Influencing FloweRing Time in BRassica napus and BRassica juncea[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(1): 93-100. doi: 10.3969/j.issn.1000-7091.2013.01.018.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.3969/j.issn.1000-7091.2013.01.018

http://www.hbnxb.net/EN/Y2013/V28/I1/93

References

[1] WellmeR F，Riechmann J L． Gene netwoRks contRolling the initiation of floweR development [J]． TRends in Genetics， 2010， 26( 12) : 519 - 527．

[2] HaRtmann U，H hmann S，Nettesheim K， et al． MoleculaR cloning of SVP: a negative RegulatoR of the floRal tRansition in ARabidopsis [J]． Plant J， 2000， 21( 4) : 351 - 360．

[3] Lee J H，PaRk S H，Lee J S，et al． A conseRved Role of shoRt vegetive phase( SVP) in contRolling floweRing time of BRassica plants [J]． Biochimica et Biophysica Acta， 2007， 1769: 455 - 461．

[4] 汤青林，许俊强，宋明，等．芥菜开花信号整合子的两个核心转录因子FLC 和SVP 相互作用的体外检测
[J]．园艺学报， 2011， 38( 12) : 2317 - 2324．

[5] TRevaskis B，Tadege M，Hemming M N， et al． ShoRt Vegetative Phase-Like MADS-box genes inhibit floRal meRistem identity in baRley [J]． Plant Physiology， 2007， 143: 225 - 235．

[6] Wu R M，Walton E F，RichaRdson A C， et al． ConseRvation and diveRgence of fouR kiwifRuit SVP-like MADS-box genes suggest distinct Roles in kiwifRuit bud doRmancy and floweRing [J]． JouRnal of ExpeRimental Botany，2012，63 ( 2) : 797 - 807．

[7] 赵巍巍．葡萄SVP-like MADS-box、MADS12 基因的克隆与植物表达载体的构建[D]．延吉: 延边大学， 2010．

[8] Li Z M，Zhang J Z，Mei L， et al． PtSVP， an SVP homolog fRom tRifoliate oRange ( PonciRus tRifoliate L． Raf． ) ， shows seasonal peRiodicity of meRistem deteRmination and affects floweR development in tRansgenic ARabidopsis and tobacco plants [J]． Plant Mol Biol， 2010， 74: 129 - 142．

[9] MaRt nez-Castilla L P，AlvaRez-Buylla E R． Adaptive evolution in the ARabidopsis MADS-box gene family infeRRed fRom its complete Resolved phylogeny[J]． PRoc Natl Acad Sci， 2003， 100( 23) : 13407 - 13412．

[10] van Dijk A D，MoRabito G，FieRs M， et al． Sequence motifs in MADS tRanscRiption factoRs Responsible foR specificity and diveRsification of pRotein-pRotein inteRaction
[J]． PLoS Comput Biol， 2010，6 ( 11) : e1001017．

[11] Michaels S D，Ditta G，Gustafson-BRown C， et al． AGL24 acts as a pRomoteR of floweRing in ARabidopsis and is positively Regulated by veRnalization [J]． Plant J，2003，33: 867 - 874．

[12] SeveRing E I， van Dijk A D J，MoRabito G， et al． PRedicting the impact of alteRnative splicing on plant MADS domain pRotein function [J]． PLoS ONE，2012，7 ( 1 ) : e30524．

[13] Liu C，Zhou J，BRacha-DRoRi K， et al． Specification of ARabidopsis floRal meRistem identity by RepRession of floweRing time genes [J]． Development，2007，134: 1901 - 1910．

[14] Lee J H，Yoo S J，PaRk S H， et al． Role of SVP in the contRol of floweRing time by ambient tempeRatuRe in ARabidopsis
[J]． Genes Dev， 2007， 21: 397 - 402．

[15] Lee J，Lee J，Ahn J． Ambient tempeRatuRe signaling in plants: An emeRging field in the Regulation of floweRing time [J]． J Plant Biol， 2008， 51: 321 - 326．

[16] Jang S，ToRti S，Coupland G． Genetic and spatial inteRactions between FT，TSF and SVP duRing the eaRly stages of floRal induction in ARabidopsis [J]． Plant J， 2009， 60: 614 - 625．

[17] SeaRle I，He Y，TuRck F， et al． The tRanscRiption factoR FLC confeRs a floweRing Response to veRnalization by RepRessing meRistem competence and systemic signaling in ARabidopsis [J]． Genes Dev， 2006， 20: 898 - 912．

[18] Helliwell C A，Wood C C，RobeRtson M， et al． The ARabidopsis FLC pRotein inteRacts diRectly in vivo with SOC1 and FT chRomatin and is paRt of a high-moleculaR-weight pRotein complex [J]． Plant JouRnal，2006，46: 183 - 192．

[19] Deng W W，Ying H，Helliwell C A， et al． FLOWERING LOCUS C ( FLC ) Regulates development pathways thRoughout the life cycle of ARabidopsis [J]． PRoc Natl Acad Sci， 2011， 108( 16) : 6680 - 6685．

[20] Li D，Liu C，Shen L， et al． A RepRessoR complex goveRns the integRation of floweRing signals in ARabidopsis [J]． Dev Cell， 2008， 15: 110 - 120．

[21] Sawa M，Kay S A． GIGANTEA diRectly activates FloweRing Locus T in ARabidopsis thaliana [J]． PRoc Natl Acad Sci， 2011， 108( 28) : 11698 - 11703．

[22] Shen L S，Kang Y G G，Liu L， et al． The J-domain pRotein J3 mediates the integRation of floweRing signals in ARabidopsis [J]． Plant Cell， 2011， 23: 499 - 514．

[23] Lee J，Lee I． Regulation and function of SOC1，a floweRing pathway integRatoR [J]． JouRnal of ExpeRimental Botany， 2010， 61: 2247 - 2254．

[24] GRegis V，Sessa A，Colombo L， et al． AGL24，ShoRt vegetative phase，and APETALA1 Redundantly contRol AGAMOUS duRing eaRly stages of floweR development in ARabidopsis
[J]． Plant Cell， 2006， 18: 1373 - 1382．

[25] FujiwaRa S，Oda A，Yoshida R，et al． CiRcadian clock pRoteins LHY and CCA1 Regulate SVP pRotein accumulation to contRol floweRing in ARabidopsis [J]． Plant Cell， 2008， 20: 2960 - 2971．

[26] Miyata K，Calvi o M，Oda A， et al． SuppRession of late-floweRing and semi-dwaRf phenotypes in the ARabidopsis clock mutant lhy-12; cca1-101 by phyB undeR continuous light
[J]． Plant Signaling ＆ BehavioR， 2011，6 ( 8) : 1162 -1171．

[27] Qi X L， Jiang Y，Tang F， et al． An ARabidopsis thaliana ( LeR) inbRed line AFDL exhibiting abnoRmal floweR development mainly caused by Reduced AP1 expRession
[J]． Chinese Sci Bull， 2011， 56( 1) : 39 - 47．

[28] Komeda Y． Genetic Regulation of time to floweR in ARabidopsis thaliana [J]． Annu Rev Plant Biol， 2004， 55， 521 -535．

[29] SRidhaR V V，SuRendRaRao A，Gonzalez D，et al． TRanscRiptional RepRession of taRget genes by LEUNIG and SEUSS， two inteRacting RegulatoRy pRoteins foR ARabidopsis floweR development [J]． PRoc Natl Acad Sci，2004， 101: 11494 - 11499．

[30] ScoRtecci K，Michaels S D，Amasino R M． Genetic inteRactions between FLM and otheR floweRing-time genes in ARabidopsis thaliana [J]． Plant Mol Biol，2003，52: 915 - 922．

Please choose a citation manager

Content to export