Effect on the Na+ Tolerance of Yeast Transformed with Wheat HKT Genes Family

doi:10.7668/hbnxb.2016.S1.022

Abstract

Abstract: The members of HKT family play an important role in maintaining Na⁺/K⁺ homeostasis.In order to analyze the role of wheat HKT genes in salt-tolerance mechanism,comparative analysis on Na⁺ affinity of wheat different members,the copies on different genomes and the member's alleles of wheat HKT family were conducted by transforming yeast mutant G19.Three members of HKT family, HKT1; 5, HKT2; 1,and HKT2; 2,showed different Na⁺affinity.Na⁺affinity of HKT2;2-A was higher than HKT2;2-B.Leu⁴⁸→Pro⁴⁸ mutation increased Na⁺ affinity while His²¹⁵→Tyr²¹⁵ mutation decreased that of HKT2;1.The results indicated that the two amino acids may be related to Na⁺ transporter in common wheat.

Key words: Wheat, HKT, Na⁺affinity, Yeast Na⁺ tolerance

CLC Number:

Q78
S512.03

LI Mengjun, ZHANG Shichang, XING Donghai, SHI Zhanliang. Effect on the Na⁺ Tolerance of Yeast Transformed with Wheat HKT Genes Family[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(S1): 122-126. doi: 10.7668/hbnxb.2016.S1.022.

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