Abstract: The study provided a molecular marker technology to identify the waxy foxtail millet,which could be used to utilize the excellent waxy resources of foxtail millet and to breed the waxy varieties.Firstly,through the iodine solution detection of the F₃ seeds endosperm from F₂ constructed from the hybridization of waxy foxtail millet,Shilixiang,and non-waxy foxtail millet,Yugu 1.Results showed that the ratio of plant numbers with dark blue and brownish red endosperm was 3:1,indicated that the waxy phenotype was a recessive trait controlled by a single gene.Then using waxy genotyping primers,the genomic DNA of Shilixiang was amplified by PCR and the products were sequenced.The results showed that the waxy trait was controlled by a Ⅳ type waxy gene,namely a TSI-2 transposon was inserted into the first intron of the starch synthase gene (Si006103m).Therefore,the InDel molecular markers,waxy-TSI2/int1 and waxy-int1-1F/4R,were developed based on the gene sequence of Si006103m,and then the genomic DNA of foxtail millet was amplified by them.If there was an product with 984 bp amplified by the waxy-TSI2/int1 primer and no product with 540 bp amplified by the waxy-int1-1F/4R primer,the material was identified as a waxy foxtail millet containing the type Ⅳ waxy gene.Furthermore,we identified 2 waxy materials controlled by the Ⅳ type waxy gene from 100 foxtail millet resources using two pairs of waxy primers.Therefore,the InDel markers can be used to accurately identify the Ⅳ type waxy gene from foxtail millet.

Key words: Foxtail millet, waxy gene, Molecular marker, Co-segregated

CLC Number: 

• S515.03

• S326

• Q78