Cloning and Prokaryotic Expression of TaGST from Triticum aestivum L.

doi:10.7668/hbnxb.2016.03.006

Abstract

Abstract: To investigate the function of TaGST gene,RT-PCR was used to obtain TaGST gene open reading frame sequence from wheat and analyzed by bioinformatics method.The sequence analysis results showed that the ORF of TaGST gene had a length of 690 bp coding for 229 amino acid,and the relative molecular weight of TaGST protein was 25.81 kDa and its theoretical isoelectric point was 5.29.Homology analysis showed that the amino acid sequence of TaGST was highly homologous with Oryza sativa, and phylogenetic analysis of the relationship of the newly identified TaGST with some known plant GSTs grouped the TaGST into the class of Phi GSTs.The prokaryotic expression of TaGST gene was done after construction of its prokaryotic expression vector pET32-TaGST,and the SDS-PAGE results displayed that the expressed protein was consistent with the anticipated size.The results were expected to lay a foundation for further studies on the properities and function of this gene.

Key words: Wheat, Glutathione-S-transferase, Gene clone, Porkaryotic expression

CLC Number:

Q78
S512.03

ZHANG Lei, YU Yongang, YANG Tianyou. Cloning and Prokaryotic Expression of TaGST from Triticum aestivum L.[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(3): 38-43. doi: 10.7668/hbnxb.2016.03.006.

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http://www.hbnxb.net/EN/Y2016/V31/I3/38

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