Cloning and Functional Analysis of the Lectin Gene in Pinellia peltata

doi:10.7668/hbnxb.2016.03.005

Abstract

Abstract: To clone a lectin gene from Pinellia peltata and analyze its bioactivity.A lectin gene,designated as ppl, was cloned by using RACE in Pinellia peltata .The open reading frame (ORF) of P.peltata lectin (ppl) was constructed into the pET-28a vector.A recombinant protein about 33 kDa in the Escherichia coli BL21 was induced.The purified recombinant protein was used blood coagulation experiment and anticancer experiment in vitro .The full-length cDNA of ppl was 1 504 bp,and contained an 813 bp ORF with a deduced amino acid sequence of 270 residues.The putative PPL protein contained three mannose binding site.The PPL had clotting activity,and this activity could be inhibited by mannose.The result of anticancer experiment in vitro showed that the PPL could inhibit the growth of nasopharyngeal carcinoma cell (CNE),human cervical carcinoma cell (Hela) and human breast cancer cell (Bcap-37).These results were useful for further determination of the function of P.peltata lectin protein (PPL).

Key words: Pinellia peltata, Lectin, Gene cloning, Functional analysis

CLC Number:

YANG Zaijun, LIU Lingling, PENG Zhengsong. Cloning and Functional Analysis of the Lectin Gene in Pinellia peltata[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(3): 32-37. doi: 10.7668/hbnxb.2016.03.005.

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