Cloning and Expression Analysis of Grape's Stress Inducible Promoter

doi:10.7668/hbnxb.2016.01.013

Abstract

Abstract: To study the expression of grape stress tolerance gene (CAN70200.1),a 1 354 bp promoter fragment (named as P_CAN) upstream of the gene CAN70200.1 was isolated by using PCR technology.Promoter sequence was analyzed by the database of PlantCARE and PLACE.The result showed that the P_CAN sequence contained basic elements CAAT-box,TATA-box and some cis-acting elements that response to abiotic stresses,light and plant hormones.To verify the expression pattern of the promoter,the P_CAN fragment was fused with GUS reporter gene located on pCAMBIA1391Z to construct a plant expression vector p1391Z-CAN,followed by transformation into tobacco by Agrobacterium-meditated method.The expression activity of P_CAN promoter reached highest at 120 min after drought stress treatment or at 30-60 min under 4℃ cold treatment condition,indicated that the P_CAN promoter could express under the condition of treatments with cold and drought.

Key words: Grape, P_CAN promoter, Stress induction expression, GUS assay

CLC Number:

Q78
S565.1

LÜ Zhaoyong, ZHAO Chunmei, XUE Rengao. Cloning and Expression Analysis of Grape's Stress Inducible Promoter[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(1): 77-82. doi: 10.7668/hbnxb.2016.01.013.

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