Effect of 6-BA on Organic Acid Content and Related Genes Expression in Grape Berry

doi:10.7668/hbnxb.2017.05.023

Abstract

Abstract: In order to study the effect of 6-BA on grape fruit organic acid accumulation,and the possible molecular mechanism of 6-BA regulating organic acid synthesis,a grape cultivar,Rizamat was selected to study the effect of 6-BA on fruit organic acid accumulation and related genes expression. The inflorescences and fruit clusters were treated with 6-BA at 5 days before blooming,3 and 10 days after anthesis respectively.The results showed that organic acid content of the mature grape fruit was closely related to treatment concentration of 6-BA. Tartaric acid and total organic acid content of the mature fruits that treated by 30 mg/L 6-BA was significantly lower than control. But it didn't play important roles in the content of malic acid,citric acid and oxalic acid. For 10,20 mg/L 6-BA treatment,all kinds of organic acid didn't show significant difference between treated and control mature fruits. Gene expression result showed that the expression of IDH, MDH and PEPC were mainly suppressed by 6-BA treatment,but it also promoted the expression of ME. Therefore,we speculated that the reason for inhibiting organic acid synthesis was that parts of the organic synthesis related genes expression were affected by 6-BA treatment.

Key words: Grape, 6-BA, Organic acid, Gene expression

CLC Number:

S663.03

WANG Xicheng, QIAN Yaming, WU Weimin, ZHAO Mizhen, ZHOU Beibei, WANG Zhuangwei, WU Jianhua. Effect of 6-BA on Organic Acid Content and Related Genes Expression in Grape Berry[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2017, 32(5): 149-153. doi: 10.7668/hbnxb.2017.05.023.

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