Cloning and Analysis of Dehydrin Gene NtDEH1 in Nicotiana tabacum

doi:10.7668/hbnxb.2016.01.012

Abstract

Abstract: To study the function and mechanism of dehydrin in early embryogenesis of plant,egg cells were isolated from tobacco to construct a cDNA library,and a gene named NtDEH1 were found.The full sequence of NtDEH1 were obtained by RACE,genome cloning and bioinformatics analysis showed that NtDEH1 included a 651 bp CDS region and a 535 bp intron.NtDEH1 which contained 217 amino acids were hydrophilic and acidic,the theory isoelectric point was 5.27.The amino acid homology of NtDEH1 compared Nicotiana tabacum to Nicotiana tomentosiformis,Nicotiana sylvestris,Capsicum annuum,Solanum tuberosum,Solanum lycopersicum and Salvia miltiorrhiza showed that NtDEH1 homologous sequences in all these species were conserved and belonged to SK₂ type.5' flanking sequence of NtDEH1 gene was also obtained by genome-walking,and the promoter activity of 5' flanking sequence was checked by transient gene expression.The result laid a foundation in researching the role of NtDEH1 gene in plant reproduction.

Key words: Tobacco, Dehydrin, Gene cloning, NtDEH1, Promoter, Egg cell

CLC Number:

LUO An, ZHAN Ling. Cloning and Analysis of Dehydrin Gene NtDEH1 in Nicotiana tabacum[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(1): 71-76. doi: 10.7668/hbnxb.2016.01.012.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2016.01.012

http://www.hbnxb.net/EN/Y2016/V31/I1/71

References

[1] Graether S P,Boddington K F.Disorder and function:a review of the dehydrin protein family[J].Front Plant Sci,2014,5:576.
[2] Rorat T.Plant dehydrins-tissue location,structure and function[J].Cellular & Molecular Biology Letters,2006,11(4):536-556.
[3] Wise M J.LEAping to conclusions:a computational reanalysis of late embryogenesis abundant proteins and their possible roles[J].BMC Bioinformatics,2003,4:52.
[4] Close T J.Dehydrins:A commonality in the response of plants to dehydration and low temperature[J].Physiol Plant,1997,100:291-296.
[5] Hanin M,Brini F,Ebel C,et al.Plant dehydrins and stress tolerance-versatile proteins for complex mechanisms[J].Plant Signaling and Behavior,2011,6:1503-1509.
[6] Yang Y,He M,Zhu Z,et al.Identification of the dehydrin gene family from grapevine species and analysis of their responsiveness to various forms of abiotic and biotic stress[J].BMC Plant Biol,2012,12:140.
[7] Wang Y,Xu H,Zhu H,et al.Classification and expression diversification of wheat dehydrin genes[J].Plant Sci,2014,214:113-120.
[8] Hara M,Shinoda Y,Kubo M,et al.Biochemical characterization of the Arabidopsis KS-type dehydrin protein,whose gene expression is constitutively abundant rather than stress dependent[J].Acta Physiologiae Plantarum,2011,33:2103-2116.
[9] Vaseva II,Anders I,Feller U.Identification and expression of different dehydrin subclasses involved in the drought response of Trifolium repens[J].J Plant Physiol,2014,171(3-4):213-24.
[10] Ruibal C,Salamó I P,Carballo V,et al.Differential contribution of individual dehydrin genes from Physcomitrella patens to salt and osmotic stress tolerance[J].Plant Sci,2012,190:89-102.
[11] Kim E C,Lee H S,Choi D W.Sequence variability and expression pattern of the dehydrin gene family in Populus tremula×Populus alba var.glandulosa.[J].Plant Omics,2012,5:122.
[12] Koag M C,Fenton R D,Wilkens S,et al.The binding of maize DHN1 to lipid vesicles.Gain of structure and lipid specificity[J].Plant Physiology,2003,131(1):309-316.
[13] Hara M,Fujinaga M,Kuboi T.Radical scavenging activity and oxidative modification of citrus dehydrin[J].Plant Physiology and Biochemistry,2004,42(7/8):657-662.
[14] Hara M,Fujinaga M,Kuboi T.Metal binding by citrus dehydrin with histidine-rich domains[J].Journal of ExPerimental Botany,2005,56(420):2695-2703.
[15] Arora R,Wisniewski M E.Cold acclimation in genetically related (sibling) deciduous and evergreen peach (Prunus persica L.Batsch).Ⅱ.A 60-kilodalton bark protein in cold-acclimated tissues of peach is heat stable and related to the dehydrin family of proteins[J].Plant Physiology,1994,105(1):95-101.
[16] Lopez C G,Banowetz G,Peterson C J,et al.Differential accumulation of a 24-kd dehydrin protein in wheat seedlings correlates with drought stress tolerance at grain filling[J].Hereditas,2001,135(2/3):175-181.
[17] Zhao J,Xin H,Qu L,et al.Dynamic changes of transcript profiles after fertilization are associated with de novo transcription and maternal elimination in tobacco zygote,and mark the onset of the maternal-to-zygotic transition[J].Plant J,2011,65(1):131-45.
[18] Nylander M,Svensson J,Palva E T,et al.Stress-induced accumulation and tissue-specific localization of dehydrins in Arabidopsis thaliana[J].Plant Molecular Biology,2001,45(3):263-279.
[19] Bravo L A,Close T J,Corcuera L J,et al.Characterization of an 80-kDa dehydrin-like protein in barley responsive to cold acclimation[J].Physiol Plant,1999,106:177-183.
[20] Godoy J A,Lunar R,Torres-Schumann S,et al.Expression,tissue distribution and subcellular localization of dehydrin TAS14 in salt-stressed tomato plants[J].Plant Mol Biol,1994,26:1921-1934.
[21] Ma H,Sun M,An T T,et al.Distribution,structure and function of dehydrin[J].Molecular Plant Breeding,2015,13(7):1668-1672.

Please choose a citation manager

Content to export