Cloning and Sequence Analysis of CCR Gene from Mango

doi:10.7668/hbnxb.2014.S1.004

Abstract

Abstract: In order to study the resistance function of mango of a cinnamoy-CoA reducase (CCR) gene.CCR was cloned from mango with 3'RACE and 5'RACE method.The characteristics of cDNA sequence was analyzed by software such as DNAMAN,TMHMM.The bioinformatics analysis on the obtained sequence was showed:CCR gene of mango contains coding region,3'and 5'untranslated region of length cDNA sequence of 1 292 bp,encoding 305 amino acid.The gene without transmembrance by transmembrance domains predicted.The secondary structure of CCR protein had random coil and beta helix.It was found that the gene encoding for the protein had close relationship with American aspen wood,oil-tea camellia,other distantly relationship with lily,and rubber tree through phylogenetic analysis.The CCR gene was cloned from mango and the bioinformatics analysis,laid the groundwork for the follow-up work of transgenic.

Key words: Mango, Cinnamoyl-CoA reductase (CCR), Gene cloning, Sequence analysis

CLC Number:

Q78
S667.7

ZHANG Yu, ZHANG Bo, ZHAO Zhi-chang, GAO Ai-ping, CHEN Ye-yuan, HUANG Jian-feng, DANG Zhi-guo, LUO Rui-xiong. Cloning and Sequence Analysis of CCR Gene from Mango[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2014, 29(S1): 16-19. doi: 10.7668/hbnxb.2014.S1.004.

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