Microsatellite Marker's Isolation，Primer Design and Application in the Goldfish

doi:10.7668/hbnxb.2013.S1.006

Abstract

Abstract: Microsatellite DNA was isolated from goldfish with combining biotin capture method. We obtained 38 positive clones and isolated 31 microsatellite sequences. 12 pairs of primers were designed by Primer Premier 5.0 and 8 pairs of primers can obtain amplifying products among them. The genetic diversity of the artificial breed goldfish populations were studied by using the 8 Microsatellite primers. The results showed that the genetic diversity level of two populations were high. The average heterozygosity(Ho) in two populations was from 0. 534 3 to 0. 565 5,average excepted heterozygosity(He) was from 0. 628 6 to 0. 657 0,and average polymorphism information content (PIC) was from 0. 500 9 to 0. 510 6. The genetic distance was 0. 095 7 and genetic identity was 0.921 1.All of 8 microsatellite locus could be used for genetic analysis in goldfish.

Key words: Goldfish, Microsatellite, Enrichment by magnetic beads, Genetic diversity

CLC Number:

ZHANG Sheng-li, YANG Pu, LIANG Yong-jun, SUN Xiang-jun, SUN Yan-sheng, SU Jian-tong, SHI Dong-jie. Microsatellite Marker's Isolation，Primer Design and Application in the Goldfish[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(S1): 27-31. doi: 10.7668/hbnxb.2013.S1.006.

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