Chromosome Arm Location of Powdery Mildew Resistance Gene of Common Wheat Germplasm N9134

doi:10.7668/hbnxb.2011.06.008

Abstract

Abstract: A powdery mildew resistance gene PmAS846derived from Triticum dicoccoidesaccession As846 and was located on 5B chromosome． The aim of this research was to locate this gene to chromosome arm． Genetic analysis of PmAS846transferred to common wheat line N9134 was done by ditelocentric line and SSR markers． F1 progeny between common wheat CS(Chinese Spring) disomics，CSDt5BL(CS 5BL ditelocentric line) and N9134 were highly resistant to powdery mildew． The ratio of the resistant and susceptible plants in CS /N9134 F2 progeny and CSDt5BL /N9134 / /CS BC1F1 progeny fitted the expected 3∶ 1 and 1∶ 1， respectively． The karyotype analysis of root tip cell and powdery mildew resistance for 64 CS5BL /N9134 / /CS BC1F1 plants was done，and the crossover types had frequencies of 12． The analysis results of SSR marker Xgwm67in CSN5AT5D，CSN5BT5D，CSN5DT5A，CSDt5AL， CSDt5BL and CSDt5DL indicated that the marker was absent in CSN5BT5D and present in CS Dt5BL． The results showed that PmAS846was located on 5BL and the distance of PmAS846and the 5B centromere was 18． 75 map units．

Key words: Triticum dicoccoides, Powdery mildew resistance gene, Chromosome arm location, SSR marker

CLC Number:

WANG Chang-you, WANG Ya-juan, ZHANG Hong, LIU Xin-lun, CAI Dong-ming, JI Wan-quan. Chromosome Arm Location of Powdery Mildew Resistance Gene of Common Wheat Germplasm N9134[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(6): 37-40. doi: 10.7668/hbnxb.2011.06.008.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2011.06.008

http://www.hbnxb.net/EN/Y2011/V26/I6/37

References

[1] Huang X Q，Rder M S. Molecular mapping of powdery mildew resistance genes in wheat: a review[J]. Euphytica， 2004， 137: 203-223.
[2] Blanco A，Gadaleta A，Cenci A， et al. Molecular mapping of the novel powdery mildew resistance gene Pm36 introgressed from Triticum turgidum var. dicoccoides in durum wheat[J]. Theor Appl Genet， 2008， 117: 135-142.
[3] Li G Q，Fang T L，Zhang H T， et al. Molecular identification of a new powdery mildew resistance gene Pm41 on chromosome 3BL derived from wild emmer(Triticum turgidum var. dicoccoides) [J]. Theor Appl Genet，2009， 119: 531-539.
[4] Hua W，Liu Z J，Zhu J， et al. Identification and genetic mapping of pm42，a new recessive wheat powdery mildew resistance gene derived from wild emmer(Triticum turgidum var. dicoccoides) [J]. Theor Appl Genet， 2009， 119: 223-230.
[5] Mohler V，Zeller F J，Wenzel G， et al. Chromosomal location of genes for resistance to powdery mildew in common wheat(Triticum aestivum L. em Thell.). 9. Gene MlZec1 from the Triticum dicoccoides-derived wheat line Zecoi-1[J]. Euphytica， 2005， 142: 161-167.
[6] Ahmadi H，Moore K. Inheritance and chromosomal location of powdery mildew resistance gene in wild wheat Triticum turgidum var. dicoccoides[J]. Plant Pathology Journal(Faisalabad), 2007，6(2): 164-168.
[7] Ji X L，Xie C J，Ni Z F， et al. Identification and genetic mapping of a powdery mildew resistance gene in wild emmer (Triticum dicoccoides) accession IW72 from Israel[J]. Euphytica， 2008， 159: 385-390.
[8] 王长有，吉万全，张改生，等. 小麦种质N9134 抗白粉病基因的SSR 标记和染色体初步定位[J]. 作物学报，2007， 33(1): 163-166.
[9] 韩俊，张连松，李根桥，等. 从野生二粒小麦导入普通小麦的抗白粉病基因MlWE18 分子标记定位[J].作物学报， 2009， 35(10): 1791-1797.
[10] 李根桥，房体麟，张宏涛，等. 来自野生二粒小麦IW3和IW10 的两个抗白粉病基因的鉴定及SSR 标记定位[J]. 作物学报， 2009， 35(5): 761-767.
[11] 张连松，华为，关海英，等. 野生二粒小麦导入普通小麦的抗白粉病基因MlWE29 分子标记定位[J]. 作物学报， 2009， 35(6): 998-1005.
[12] 华为，郭霞，朱婕，等. 野生二粒小麦导入普通小麦抗白粉病基因MlWE27 的鉴定和分子标记[J].农业生物技术学报， 2010， 18(1): 3-9.
[13] Zhang H T，Guan H Y，Li J T， et al. Genetic and comparative genomics mapping reveals that a powdery mildew resistance gene Ml3D232 originating from wild emmer co-segregates with an NBS-LRR analog in common wheat(Triticum aestivum L.) [J]. Theor Appl Genet， 2010， 121(8): 1613-1621.
[14] Ben-David R，Xie W L，Peleg Z， et al. Identification and mapping of PmG16，a powdery mildew resistance gene derived from wild emmer wheat[J]. Theor Appl Genet， 2010， 121: 499-510.
[15] 吉万全，王秋英，薛秀庄，等. 野生二粒小麦抗白粉病基因的转移及其RAPD 分析[J]. 西北植物学报，1999， 19(6): 31-36.
[16] 王秋英，吉万全，王长有，等. 小麦抗白粉病种质“N9134”的抗性遗传分析[J]. 西北农林科技大学学报: 自然科学版， 2004， 32(2): 14-16.
[17] 盛宝钦. 用反应型记载小麦苗期白粉病[J]. 植物保护， 1988(1): 49.
[18] 鲁玉柱，薛秀庄，王长有，等. 应用RAPD 标记检测导入普通小麦的Elymus rectisetus 遗传物质[J]. 西北植物学报， 2002， 22(1): 51-55.
[19] Rder M S，Korzun V，Wendekhake K， et al. A microsatellite map of wheat[J]. Genetics，1998，149: 2007-2023.
[20] 桑利群，王长有，王秋英，等. 小麦种质N9659 抗白粉病基因SSR 标记研究[J]. 华北农学报， 2008， 23(3):166-169.
[21] 王长有，王秋英，刘志立，等. 小麦新种质N95175 抗白粉病基因的染色体定位[J]. 华北农学报， 2010， 25(1): 217-220.

Please choose a citation manager

Content to export