Cloning of Wheat TaWRKY46-1 Gene and Construction of Plant Inducible Expression Vector by Gateway Cloning Technique

doi:10.7668/hbnxb.2011.02.004

Abstract

Abstract: In this study, the wheat variety, Y anon ai 158(Triticun aestiven L),was used as the experinentalm,terial for cloning the wheat TaWRK Y46 gene Primers were designed based on a known wheat TaWRKY46 gene sequence in NCBI, subsequently an 870 bp cDNA fragment was amplified from total RNA of Yarm ai 158 by RT-PCR Sequence analysis revealed that the cloned fragment contains the full-length cDNA and is highly hologous to the TaWRKY46 sequence in NCBI The sequence alignment revealed a Q to E change with in the conserved "WRKYGQK" domain, suggestilg it was a new allele of TaWRKY46. Hencewe designated it asTaWRKY46-1. In onler to e1uciclate liznctional role of the new Yangnai 158TaLYRKY46-1 allele, a plant ioucible over expression vector, pKIGW-TaWRKY 46-1, was constmcted via Gatrway cloning technology and tr-anslonned intoAgroFxzcteriun cells successiiilly Thiswmk proviles the key basis for future detailed functional analysis of wheat TaWRKY46-1 gene.

Key words: Wheat, WRKY transcription factor, Gateway cloning technology, Plant inducible expression vector

CLC Number:

LI Ming, DING Bo, NIU You-xiong, LU Fang-fang, YANG Wen-li, Silin Zhong, SUN Shou-jun, XIE Xiao-dong. Cloning of Wheat TaWRKY46-1 Gene and Construction of Plant Inducible Expression Vector by Gateway Cloning Technique[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(2): 17-22. doi: 10.7668/hbnxb.2011.02.004.

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