Genetic Diversity of Disease-Specific Protein Gene from Rice Stripe Virus in Laodelphax striatellus

doi:10.7668/hbnxb.2010.06.025

Abstract

Abstract: The genetic diversity of disease-specific protein(sp)from twenty-one isolates of Rice striPe virus in Laodelphax striateUus collected from Jiangsu, Yunnan, Shandong and Hebei provinces was analyzed.Sequence analysis results showed that the cDNA include 537 bp of sp gene and encode 178 amino acids and a stop codon.By DNAStar software, the similarities of nucleotide and deduced amino acid sequences of the sp genes among these isolates were 96.1%to 100%and 95.0%to 99.4%.respectively.The nucleotide sequences obtained in the study were compared with previously reported sequences of other 27 isolates in rice.Results demonstrated that the nucleotide of RSV-sp gene had larger molecular variation, and had no obviously regularity except that the isolates from Laodelphax striateUus and Yunnan belonged to an independent group.Functional analysis revealed that the genetic diversity of RSV-sp gene was related to host first which was divided into two hosts colonies：Laodelphax striatellus and rice.In addition, it was correlated with area secodenly which was divided into two geographical colonies in Laodelphax striatellus colony and many geographical colonies in rice colony：Yunnan in China, the rests without Yunnan in China.

Key words: Rice stripe virus, Disease—specific protein gene, Genetic diversity, Hosts colony, Geographical colony

CLC Number:

S435.111.4

REN Chun-mei, CHENG Zhao-bang, JI Ying-hua, Xiong Ru-yi, FAN Yong-jian, ZHOU Yi-jun. Genetic Diversity of Disease-Specific Protein Gene from Rice Stripe Virus in Laodelphax striatellus[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(6): 132-138. doi: 10.7668/hbnxb.2010.06.025.

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