Screening and Identification of a Facultative Anaerobic Cellulase-producing Strain and Characterization of Cellulase

doi:10.7668/hbnxb.2010.06.026

Abstract

Abstract: Strain CR.14 producing extacellular cellulase was isolated from contents of cattal lumen.According to its morphological, the physiological and biochemical properties, 16 S rDNA phylogenetic analysis, indicated that the strain belonged to CeUoulonmonas sp.e main components of eellulase of strain CR一14 was C_x, C₁ and B-glucosidase. -11le activity of C。WaS the highest, while activity of C_l and B—glucosidase Was relatively low.r11Ile optimal enzyme reaction conditions Was 50℃.pH 6.0.Under the conditions of 60℃and pH 6.0—7.5 cellulase Was stability.Mn²⁺, Fe²⁺could significantly promote the vitality of the enzyme.Zn²⁺, Cu²⁺, Ca²⁺and K+inhibit the vitality of the enzyme.

Key words: Cattal ramen, Facultative anaerobic, Identification, Celloulonmonas

CLC Number:

TQ925

ZHANG Li-ping, LI Ya-bing, CHENG Hui-cai, HUANG Ya-li, ZHANG Ning. Screening and Identification of a Facultative Anaerobic Cellulase-producing Strain and Characterization of Cellulase[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(6): 139-143. doi: 10.7668/hbnxb.2010.06.026.

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