Relationship of Iris Inferred from the Chloroplast DNA trnL-F Sequence Variation

doi:10.7668/hbnxb.2010.05.023

Abstract

Abstract: The intergenic region of trnL and trnF was amplified by PCR for 24 samples of Iris. The lengths of the segments varied from 1 003 bp to 1 113 bp,of which 954 bp was compared. The number of the evolutionary loci was 106,including 92 transpositional loci and 14 missing ones,which was 11. 11% of the compared lengths. Their relation-ships were performed using software DNAMAN. It showed that all the samples were divided into two parts. The first part included two groups,one was I. lactea from Taipu,Xiwuqi and Wulumuqi,the other included I. tigridia,I. sanguinea and I. halophila. The second part included two groups. One group included I. lactea from Beijing,Minqin, Guyuan,Taishan,Wuwei and Zhuozhou,I. dichotoma and I. proantha. The other group included I. tectorum,I. collettii, I. germanica,I. pumila,I. dichotoma,I. wattii,I. foetidissima and I. ungiucularis.

Key words: Iris, Relationship, Chloroplast DNA( cpDNA), TrnL-F sequence

CLC Number:

S68.03

MU Shaohua, SUN Zhenyuan, PENG Zhenhua. Relationship of Iris Inferred from the Chloroplast DNA trnL-F Sequence Variation[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(5): 112-116. doi: 10.7668/hbnxb.2010.05.023.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2010.05.023

http://www.hbnxb.net/EN/Y2010/V25/I5/112

References

[1] 中国科学院中国植物志编辑委员会.中国植物志[M].第16卷,第1分册.北京:科学出版社,1985.

[2] 刘云.吉林省八种鸢尾植物的RAPD分析及结构植物学分析[D].哈尔滨:东北师范大学,2001:45.

[3] 黄芸,杨光.RAPD法鉴定射干类中药[J].中草药,2002,33(10):935-937.

[4] 秦民坚,黄芸,杨光,等.射干及类似药用植物叶绿体rbcL基因序列分析[J].药学学报,2003,38(2):147-152.

[5] 胡金勇,曾英,桑玉英.双向电泳分析鸢尾绿白嵌合叶片的蛋白质[J].云南植物研究,2002,24(3):387-391.

[6] 黄瑶.叶绿体DNA及其在植物系统学研究中的应用[J].植物学通报,1994,11(2):11-25.

[7] 卢孟柱,谢红丽,张辉,等.利用叶绿体DNA变异研究胡杨系统发育[J].西北植物学报,2000,20(6):1148-1154.

[8] 汤陵华,孙加祥,宇田津撤朗,等.太湖地区水稻地方品种籼粳分类方法的比较[J].江苏农业学报,2003,19(3):139-144.

[9] Taberlet P,Gielly L.Universal primers for amplification of three non-coding regions of chloroplast DNA[J].Plant Mol Biol,1991,17:1105-1109.

[10] 卢孟柱,谢红丽,张辉,等.利用叶绿体DNA变异研究胡杨系统发育[J].西北植物学报,2002,20(6):1148-1154.

[11] Thompson J D,Gibson T J.The Clustal-X windows interface:flexible strategies for multiple sequence alignment aided by quality analysis tools[J].Nucleic Acids Research,1997,25:4876-4882.

[12] Felsenstein J.Confidence limits on phylogenies:an approach using the bootstrap[J].Evolution,1985,39:783-791.

[13] Fernandes I A,Aguilar J F,Panero J L,et al.A phylogenetic analysis of Doronicum (Asteraceae,Senecioneae)based on morphological,nuclear ribosomal(ITS)and chloroplast (trnL-F)evidence[J].Molecular Phylogenetics and Evolution,2001,20:41-64.

[14] Garcia J N,Garnatje T,Susanna A,et al.Tribal and subtribal delimitation and phylogeny of the Cardueae(Asteraceae):a combined nuclear and chloroplast DNA analysis[J].Molecular Phylogenetics and Evolution,2002,22:51-64.

[15] Garcia J N,Susanna A,Mozaffarian V,et al.Generic delimitation and phylogeny of the subtribe Centraureinae(Asteraceae):a combined nuclear and chloroplast DNA analysis[J].Annals of Botany,2001,87:503-515.

[16] Garcia J N,Susanna A,Mozaffarian V,et al.The natural delimitation of Centaurea (Asteraceae:Cardueae):ITS sequence analysis of the Centaurea jaceagroup[J].Plant Systematics and Evolution,2000,223:185-199.

[17] Vilatersana R,Susanna A,Garcia N,et al.Genetic delimitation and phylogeny of the Carduncellus-Cartharrus complex(Asteraceae)based on ITS sequences[J].Plant Systematics and Evolution,2000,221:89-105.

[18] 谢航.中国鸢尾有关分类群的讨论及属下分类系统的修订[D].哈尔滨:东北师范大学,1996.

[19] Demesore B,Sodzi N,Petit R P.A set of universal primers for amplification of polymorphic non-coding regions of mitochondrial and chloroplast DNA in plants[J].Mol Eol,1995,4:129-131.

[20] 田欣,李德铢.DNA序列在植物系统学研究中的应用[J].云南植物研究,2002,24(2):170-184.

Please choose a citation manager

Content to export