Construction of Expression Vector of the Triticum aestivum L.TaNCED1 Gene

doi:10.7668/hbnxb.2008.04.018

Abstract

Abstract: In this study, Triticum aestivum L 1 9-Cis-Epoxycarotenoid Dioxygenase gene ( TaNCED1 ) was amplified using specific primers containing restriction enzyme site of Kpn I and Xba I.PCR products and the binary expression vector pCAMBIA2300 ～ 35Swere digested by the corresponding restricted enzymes respectively,and linked directionally.The re-sulting construction with TaNCED1 was named pTaNCED1.The pTaNCED1 was further introduced into Agrobacterium tumefaciens strain G V3101 for the following-up research such as gene functional identification and gene transformation for wheat stress tolerance improvement.

Key words: Triticum aestivun L, 9-Cis-Epoxycarotenoid Dioxygenase(NCED), ABA

CLC Number:

Q786

XI Ting-ye, WANG Hong-gang, KOU Meng, LIU Hai-yan, LIU Shu-bing. Construction of Expression Vector of the Triticum aestivum L.TaNCED1 Gene[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(4): 77-80. doi: 10.7668/hbnxb.2008.04.018.

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