Effect of Some Plant Growth Regulators Used by Stages Treatments on Endosperm Cell and Starch Granule in Maize Endosperm Development

doi:10.7668/hbnxb.2018.06.019

Abstract

Abstract: In order to study the effect of exogenous plant growth regulators on maize grain filling, maize inbred line B73 was used by some approaches such as cell counting and starch granule particle analysis to analyze the process of endosperm development of kernels. The main work focused on the development of grain filling, endosperm cells and starch granules after ethephon treatment in jointing stage and in combination with some plant regulators in silking stage. It was found that the grain filling rate in DAP 20 dropped by almost 17.7% in the ethephon treated plant, the 100-kernel weight in the ethephon treated plant decreased by 11.3% in contrast to the control plant in DAP 44. The endosperm cell number in the ethephon treated plant decreased significantly 22.9% compared with the control plant in DAP 12 and the starch granule number with the endosperm area of IV, V and VI in ethephon treated plant dropped notably. The starch granule diameter distribution in the ethephon treated plant (Treatment B) changed significantly in different stages after pollination compared with the control plant. The endosperm cell number and starch granule distribution showed nearly no difference in ethylene treatment in combination with abscisic acid treatment (Treatment E) in silking stage and in treatment B. Compared with the treatment B, the 100-kernel weight, the grain filling rate, the starch granule diameter distribution and the starch granule number per endosperm were all improved in some degree after ethylene treatment and with the compounding of 1-MCP (Treatment C) or 6-BA(Treatment D). The 100-kernel weight and the starch granule number per endosperm in treatment D were consistent with the control plant. This experiment provided a foundation for further research on exogenous plant growth regulators used in maize.

Key words: Maize, Ethephon, Endosperm cell, Starch granule, 1-MCP, 6-BA, ABA

CLC Number:

S513.01

LIU Xiaoqing, ZHAN Yanting, WANG Yue, WANG Ping, YIN Shikai, HAN Caixia, DONG Xuehui. Effect of Some Plant Growth Regulators Used by Stages Treatments on Endosperm Cell and Starch Granule in Maize Endosperm Development[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2018, 33(6): 137-144. doi: 10.7668/hbnxb.2018.06.019.

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