Screening of Wheat Genotype Sensitive by Agrobacterium tumefaciens

doi:10.7668/hbnxb.2008.04.019

Abstract

Abstract: The development of Agrobacterium -mediated transformation of wheat are restricted because the short of wheat genotype sensitive. This paper uses the method of the transformation expression of gus gene and PCRmeasure, de - tected 68 different materials of mature embryos in transformation expression of gus and 8 different materials of plants which were transformed from immature embryos, the results showed that foreign genes can be detect in Yangmai10, Shilu - an02 -1, Shijiangzhuang5, Zhoumai18, Shangdong01 -35, Taishan008, Y9 -63and Handan3475, so they are sensitive geno - type materials and can be used for the receptor of the Agrobacterium infection; Gus genes were expressed in Yangmai10 and Shiluan02 -1, the best effects of infection were resuming training in 14 The embryos members of Gus gene expres - sion were increased, and the volume of locus caeruleus were augmented?So Yangmai10 and Shiluan02 -1 can be used for the materials of the grading -up system.

Key words: Agrobacterium, Genotype sensitive, Wheat, Gus, PCR

CLC Number:

S512
Q943

GUO Zhi-jiang, DING Zai-song, WANG Jin-ming, ZHAO Ming. Screening of Wheat Genotype Sensitive by Agrobacterium tumefaciens[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(4): 81-84. doi: 10.7668/hbnxb.2008.04.019.

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