Proteomic Analysis of Embryonic Dormancy in Wheat

doi:10.7668/hbnxb.2009.02.001

Abstract

Abstract: A proteomic approach was used to analyze the mechanisms of dormancy breaking in wheat embryos and the participation of hydrogen peroxide (H₂O₂) and abscisic acids (ABA) in this process. The dormant and non-dormant embryos were dissected and treated with water,1 % H₂O₂ and 25μmol/ L ABA solution. ABA delayed,whereas H₂O₂ promoted seed dormancy breaking. 2D maps for water,ABA and H₂O₂ were established. Ten spots,showing significant changes in expression level,were identified by MALDI2TOF2MS. Some of them had previously been shown to play a role during dormancy breaking or germination in several plant species.We conclude that the mechanism of seed dormancy breaking is acomplex developmental processes,involves in environment stress (such as LEA,NAD(P) H dehydrogenase),cell cycle activity(auxin-responsive protein IAA19),signal transduction (calmodulin2related protein) and seed storage protein (2S albu-min-like protein),etc.Altogether,the results provided important information on the molecular mechanisms of breaking dormancy in the wheat seeds by H₂O₂ application.

Key words: Wheat, Dormancy, ABA, Hydrogen peroxide, Proteome

CLC Number:

S512.03

SUN Guo-zhong, ZHANG Xiu-ying, YAN Chang-sheng, XIAO Shi-he. Proteomic Analysis of Embryonic Dormancy in Wheat[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(2): 1-7. doi: 10.7668/hbnxb.2009.02.001.

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